| 壬基酚对牡蛎血淋巴细胞的DNA损伤 |
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| 引用本文： | 李正炎,卫东.壬基酚对牡蛎血淋巴细胞的DNA损伤[J].癌变．畸变．突变,2006,18(6):469-471. |
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| 作者姓名： | 李正炎 卫东 |
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| 作者单位： | 中国海洋大学海洋环境与生态教育部重点实验室；中国海洋大学环境科学与工程学院 |
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| 基金项目： | 国家自然科学基金项目(No.40306019)，山东省环境保护科技项目(No.2004049) |
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| 摘 要： | 【摘要】 背景与目的： 采用单细胞凝胶电泳技术研究壬基酚(nonylphenol, NP)对牡蛎血淋巴细胞DNA的损伤作用。 材料与方法：分别以0(阴性对照)、0.025、0.10、0.25和1.00 mg/L的NP处理牡蛎24 h和48 h,检测NP对血淋巴细胞DNA的损伤作用。 结果： 当NP在0.025 mg/L时可以使牡蛎血淋巴细胞产生轻微的DNA损伤, 染毒24 h后DNA破坏率为6％，48 h后破坏率为20％。随着NP浓度的增加和染毒时间的延长，血淋巴细胞的DNA破坏率相应增加,其中最高浓度的处理组(1.00 mg/L)DNA 损伤最为明显, 染毒24 h后的破坏率为30％，48 h后为60％。 结论： NP对牡蛎血淋巴细胞具有遗传毒性。
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| 关 键 词： | 单细胞凝胶电泳 壬基酚 牡蛎 DNA损伤 遗传毒性 血淋巴细胞 |
| 文章编号： | 1004-616X（2006）06-0469-03 |
| 收稿时间： | 2005-09-05 |
| 修稿时间： | 2006-01-16 |
DNA Damage Caused by Nonylphenol in Oyster Hemolymph Cells |
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| LI Zheng-yan,WEI Dong.DNA Damage Caused by Nonylphenol in Oyster Hemolymph Cells[J].Carcinogenesis,Teratogenesis and Mutagenesis,2006,18(6):469-471. |
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| Authors: | LI Zheng-yan WEI Dong |
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| Institution: | (1.Key Lab of Marine Environmental Science and Ecology,Ministry of Education,Ocean University of China,Qingdao 266003，Shandong;2.Faculty of Environmental Science ＆ Engineering, Ocean University of China, Qingdao 266003，Shandong,China) |
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| Abstract: | BACKGROUND & AIM: The effect of nonylphenol(NP) on DNA damage in oyster hemolymph cells was measured by single-cell gel electrophoresis assay in vivo. MATERIAL AND METHODS: Oysters were exposed to various NP concentrations (0, 0.025, 0.10, 0.25 and 1.00 mg/L) and DNA damage in hemolymph cells was assessed after 24 h and 48 h. RESULTS: NP at 0.025 mg/L caused slight damage to cellular DNA. The percentage of cellular damage was 6% and 20% after 24 h and 48 h, respectively. NP at 1.00 mg/L caused serious damage to cellular DNA. The percentage of cellular damage was 30% and 60% after 24 h and 48 h, respectively. CONCLUSION: NP causes genetic toxicity to oyster hemolymph cells. |
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| Keywords: | single-cell gel electrophoresis nonylphenol oyster DNA damage genetic toxicity hemolymph cell |
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