| gp350胞外段基因表达载体的构建与表达 |
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| 引用本文： | 杨桦,王昶,郑萍,镡旭民,李红,邓安春,缪书卉,杨菲.gp350胞外段基因表达载体的构建与表达[J].重庆医学,2009,38(6). |
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| 作者姓名： | 杨桦 王昶 郑萍 镡旭民 李红 邓安春 缪书卉 杨菲 |
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| 作者单位： | 第三军医大学新桥医院耳鼻咽喉科,重庆,400037 |
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| 摘 要： | 目的 构建gp350与C3d融合基因表达载体,并在体外进行表达和鉴定.方法 以pGEX-gp350为模板,经PCR获得长846bp的gp350胞外段(25-870),将该扩增片段插入pSG.SS.C3d3.YL载体,构建pSG.SS.gp350.C3d3.YL表达质粒.经限制性内切酶鉴定和DNA序列测定证实后,将该质粒转染Hela细胞,检测其体外表达.结果 免疫细胞化学分析结果表明转染细胞有目的 分子的表达. 结论 构建的重组载体可在真核细胞内正确表达,这为基于gp350的鼻咽癌预防性疫苗下一步的动物实验奠定了基础.
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| 关 键 词： | 真核表达 鼻咽癌 |
Construction and expression of gp350 eukaryotic expression vector |
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| YANG Hua,WANG Chang,ZHENG Ping,et al..Construction and expression of gp350 eukaryotic expression vector[J].Chongqing Medical Journal,2009,38(6). |
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| Authors: | YANG Hua WANG Chang ZHENG Ping |
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| Institution: | YANG Hua,WANG Chang,ZHENG Ping,et al.(Department of Otorhinolaryngology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China) |
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| Abstract: | Objective To construct the eukaryotic expression vector for gp350 and C3d fusion gene and to express it in vitro.Methods gp350 was amplified by PCR using pGEX-gp350 as a template,and the PCR product was inserted into eukaryotic expression vector pSG.SS.C3d3.YL.The recombinant plasmid was transfected into Hela cells and the expressed product was detected by immunocytochemistry.Results Immunocytochemistry demonstrated that gp350 existed in the cytoplasm of Hela cells transfected by recombinand plasmid.Conclus... |
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| Keywords: | gp350 |
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