正交试验法优选熄风通脑胶囊提取工艺

目的:优选熄风通脑胶囊的提取工艺.方法:以芍药苷提取量和浸膏得率为指标,选取加水量、浸泡时间、提取时间和提取次数为考察因素,采用正交试验法优选熄风通脑胶囊中赤芍等10味饮片的水提取工艺；以浸膏得率为指标,选取乙醇体积分数、乙醇用量、浸泡时间、回流时间为考察因素,采用正交试验法优选熄风通脑胶囊中三七的醇提取工艺.结果:最佳水提工艺为加10倍量水浸泡0.5h煎煮2次,每次1.Sh;最佳醇提工艺为加6倍量70％乙醇浸泡0.5h,回流提取3次,提取时间分别为1.5,1,1h.结论:优选的提取工艺稳定可行,可推广于大生产应用.

Optimization of Extraction Process for Xifeng Tongnao Capsules by Orthogonal Test

Objective:To optimize extraction process of Xifeng Tongnao capsules. Method: With the content of paeoniflorin and yield of extract as indexes,the amount of water,soaking time,extraction time and extraction times were chosen as factors,orthogonal design was used to optimize water extraction process of Radix Paeoniae Rubra and nine other Chinese herbal pieces from Xifeng Tongnao capsules;With yield of extract as index,the amount and concentration of ethanol,soaking time and reflux extraction time were selected as factors,orthogonal design was used to optimize alcohol extraction process of Radix Et Rhizoma Notoginseng from Xifeng Tongnao capsules. Result: Optimum water extraction technology was as following:soaked 0.5 h with 10 times the amount of water,extracted 2 times, 1.5 hours per time;Optimum alcohol extraction process was as following:soaked 0.5 h with 6-fold of 70% ethanol,reflux extracted 3 times with extraction time of 1.5,1,1 h. Conclusion: This optimized extraction technology was stable and reliable,it could be extended to large-scale production application of Xifeng Tongnao capsules.