中心组合设计-效应面法优选化瘀祛痰颗粒的提取工艺

目的:采用中心组合设计-效应面法优选化瘀祛痰颗粒的提取工艺.方法:采用水煎法提取化瘀祛痰颗粒中有效部位,以丹酚酸B含量为指标,考察提取次数、溶媒用量、提取时间对丹酚酸B含量的影响,并对各因素的水平进行二项式拟合,通过响应面分析确定最佳工艺条件.结果:最佳提取工艺为加10倍量水提取3次,每次1.5h.丹酚酸B含量的实测值(1.027％)与理论值(1.029％)偏差较小.结论:中心组合设计-效应面法可用于复方提取工艺的优选,优选的工艺稳定可行,建立的数学模型预测性良好.

Optimization of Extraction Technology of Huayu Qutan Granules by Central Composite Design-Response Surface Methodology

Objective:To optimize extraction technology of Huayu Qutan granules by central composite design-response surface methodology. Method: Effective parts of Huayu Qutan granules was extracted by decoction,with the content of salvianolic acid B as index,extraction times,ratio of solid to liquid and extraction time as independent variables,nonlinear mathematic models were used to estimate the relationship between independent and dependent variables,response surface analysis were delineated according to these mathematic models,and optimum technology conditions were selected. Result: Optimum extraction technology was as following:extracted 3 times with 10 times the amount of water,1.5 h each time.RSD between the measured value(1.027%) and the theoretical value(1.029%) of the content of salvianolic acid B was small. Conclusion: Central composite design-response surface methodology was successfully used to optimize extraction technology of compounds,optimized technology was stable and feasible,established mathematical model had good predictability.