| 端粒酶抑制因子PinX1基因在鼻咽癌细胞中的表达及作用效应分析 |
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| 引用本文： | 赖肖芬,申聪香,文忠,钱宇虹.端粒酶抑制因子PinX1基因在鼻咽癌细胞中的表达及作用效应分析[J].中国耳鼻咽喉颅底外科杂志,2011,17(3):161-166. |
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| 作者姓名： | 赖肖芬 申聪香 文忠 钱宇虹 |
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| 作者单位： | 南方医科大学附属珠江医院耳鼻咽喉头颈外科; |
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| 基金项目： | 广东省自然基金资助(项目编号91515051501000061) |
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| 摘 要： | 目的 探讨PinX 1基因在鼻咽癌细胞中的表达及其作用效应分析.方法 构建包含全长人PinX 1基因序列的质粒载体pEGFP-C 3-PinX 1及Pinx 1的小干扰RNA,PinX1-FAMsiRNA.脂质体法转染人鼻咽癌5～8F细胞,采用RT-PCR检测PinX 1基因和端粒酶催化亚单位( hTERT) mRNA...
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| 关 键 词： | 鼻咽癌 PinX1基因 小干扰RNA 端粒酶 凋亡 |
Expression and effective analysis of telomerase inhibitor PinX1 in nasopharyngeal carcinoma cells |
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| LAI Xiao-Fen,SHEN Cong-Xiang,WEN Zhong,JIAN Yu-Gong.Expression and effective analysis of telomerase inhibitor PinX1 in nasopharyngeal carcinoma cells[J].Chinese Journal of Otorhinolaryngology-skull Base Surgery,2011,17(3):161-166. |
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| Authors: | LAI Xiao-Fen SHEN Cong-Xiang WEN Zhong JIAN Yu-Gong |
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| Institution: | LAI Xiao-fen,SHEN Cong-xiang,WEN-zhong,et al.(Department of Otolaryngology-Head and Neck Surgery,Pearl River Hospital,Southern Medical University,Guangzhou,Guangdong 510282,China) |
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| Abstract: | Objective To investigate the expression of PinX1 gene and its functional effects in human nasopharyngeal carcinoma(NPC) cells.Methods Expression vectors of human PinX1(pEGFP-C3-PinX1) and its small interfering RNA(PinX1-FAM-siRNA) were constructed and transfected into NPC 5-8F cells by lipofectamine TM 2000.First,mRNA level of PinX1 was examined with RT-PCR.And then,its effects on mRNA level of telomerase catalytic subunit(hTERT),telomerase activity,cell proliferation and apoptosis were examined using semi-... |
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| Keywords: | Nasopharyngeal carcinoma PinX1 gene siRNA Telomerase Apoptosis |
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