| 贵州葛根高效液相色谱指纹图谱研究 |
| |
| 引用本文： | 陈添福,杨玉琴,张丽艳,钟正灵,张亚洲.贵州葛根高效液相色谱指纹图谱研究[J].中国药业,2008,17(3):10-11. |
| |
| 作者姓名： | 陈添福 杨玉琴 张丽艳 钟正灵 张亚洲 |
| |
| 作者单位： | 贵阳中医学院,贵州,贵阳,550002 |
| |
| 摘 要： | 目的应用高效液相色谱（HPLC）法对贵州不同产地葛根药材进行指纹图谱研究。方法色谱柱为XDB-C18柱（150mm×4．6mm，5μm），流动相为甲醇-0．2％冰醋酸水溶液，二元梯度洗脱，流速为0.5mL／min，检测波长为250nm。结果根据选择的色谱条件制订了贵州不同产地葛根药材的指纹图谱。结论HPLC法操作简便、结果稳定、重现性好，可更好地控制葛根药材的内在质量。
|
| 关 键 词： | 葛根 高效液相色谱法 指纹图谱 |
| 文章编号： | 1006-4931（2008）03-0010-02 |
| 收稿时间： | 2007-09-19 |
| 修稿时间： | 2007-11-15 |
Fingerprint of Radix Puerariae Thomsonii by HPLC |
| |
| Chen Tianfu,Yang Yuqin,Zhang Liyan,Zhong Zhengling,Zhang Yazhou.Fingerprint of Radix Puerariae Thomsonii by HPLC[J].China Pharmaceuticals,2008,17(3):10-11. |
| |
| Authors: | Chen Tianfu Yang Yuqin Zhang Liyan Zhong Zhengling Zhang Yazhou |
| |
| Institution: | (Guiyang College of Traditional Chinese Medicine Guiyang, Guizhou, China 550002) |
| |
| Abstract: | Objective To study a fingerprint of raw material of radix purerariae thomsonii from various habitats by HPLC. Methods XDB- C18 column （150 mm×4.6 mm,5 μm） was used,with the mixture of methyl alcohol and 0.2% glacial acetic acid as the mobile phase in a gradient mode. The flow rate was 0.5 mL/min and the wavelength was 250 nm. Results According to the optimum chromatographic conditions, a good fingerprint of radix puerariae thomsonii was described. Conclusion The method is simple and accurate with good reproducibility. It provides a scientific basis for controlling the quality of radix puerariae thomsonii. |
| |
| Keywords: | radix puerariae thomsonii HPLC fingerprint |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
