| 人肺腺癌A549/DDP耐药细胞减弱顺铂诱导的细胞凋亡 |
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| 引用本文： | 刘文哲,胡义德,李金瀚. 人肺腺癌A549/DDP耐药细胞减弱顺铂诱导的细胞凋亡[J]. 癌症, 2000, 19(4): 325-327 |
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| 作者姓名： | 刘文哲 胡义德 李金瀚 |
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| 作者单位： | [1]广州赤岗一七七中心医院肿瘤科 [2]第三军医大学新桥医院全军呼吸研究所 |
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| 摘 要： | 目的：探讨人肺腺癌Ａ５４９／ＤＤＰ耐药细胞的多药抗药机理。方法；应用形态学观察、琼脂糖凝胶电泳、原位ＤＮＡ断裂点的末端标记法观察细胞亡的状况，用免疫组化法检测ｂｃｌ－２基因的表达。结果：癌细胞在顺铂作用下产生了典型的凋亡形态学改革。３μｇ／ｍｌ，５μｇ／ｍｌ，７μｇ／ｍｌ顺铂作用４８小时，Ａ５４９细胞ＤＮＡ裂解片断呈现典型的阶梯状排列条喧，而在５μｇ／ｍｌ、７μｇ／ｍｌ顺铂作用４８小时，Ａ５４９／
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| 关 键 词： | 多药抗药性 顺铂 细胞凋亡 肺腺癌 A549／DDP |
| 文章编号： | ： |
Cisplatin-induced apoptosis was reduced in cisplatin-resistant human lung adenocarcinoma cell line A549 |
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| LIU Wen zhe ,HU Yi de ,LI Jin han ,et al Oncology Department, Central Hospital PLA,Guangzhou ,P R China Institute of Respiratory Disease,Xinqiao Hospital,Third Military Medical University,Chongqing ,P R. Cisplatin-induced apoptosis was reduced in cisplatin-resistant human lung adenocarcinoma cell line A549[J]. Chinese journal of cancer, 2000, 19(4): 325-327 |
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| Authors: | LIU Wen zhe HU Yi de LI Jin han et al Oncology Department Central Hospital PLA Guangzhou P R China Institute of Respiratory Disease Xinqiao Hospital Third Military Medical University Chongqing P R |
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| Affiliation: | LIU Wen zhe 1,HU Yi de 2,LI Jin han 3,et al 1 Oncology Department,177 Central Hospital PLA,Guangzhou 510317,P R China 2 Institute of Respiratory Disease,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,P R |
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| Abstract: | |
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| Keywords: | Lung cancer Multidrug resistance Cisplatin Apoptosis bcl 2 gene |
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