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| 冰糖草总黄酮含量测定及显色方法优化 | |
| 引用本文： | 马雯芳,邓慧连,蔡毅,余娇.冰糖草总黄酮含量测定及显色方法优化[J].中国实验方剂学杂志,2013,19(3):112-115. |
| 作者姓名： | 马雯芳 邓慧连 蔡毅 余娇 |
| 作者单位： | 1. 广西中医药大学,南宁 530001;成都中医药大学,成都 610075 2. 广西中医药大学,南宁,530001 3. 广西中医药大学,南宁 530001;浙江省安吉县妇幼保健院药剂科,浙江安吉 313300 |
| 基金项目： | 广西壮族自治区食品药品监督管理局面上项目;广西壮族自治区壮药质量标准(第二册)(mzy2010002);广西自然科学基金创新研究团队项目(2011GXNSFF018006);广西科学研究与技术开发计划项目(桂科能10100027-1) |
| 摘 要： | 目的:建立用紫外-可见分光光度法测定冰糖草总黄酮含量的方法.方法:对显色剂的种类、浓度与用量进行考察,采用NaNO2-Al(NO3)3-NaOH显色反应体系,测定冰糖草中总黄酮含量.结果:以芦丁为对照品,测定波长为506 nm,在4.8～96.2 mg·L-1线性关系良好(r =0.999 3),平均回收率为103.71％ (RSD 2.68％,n=6).结论:该方法简便、快捷、准确,可作为冰糖草总黄酮含量测定的方法. |
| 关 键 词： | 冰糖草 总黄酮 含量测定 显色方法 优化 |
| 收稿时间： | 3/7/2012 12:00:00 AM |
Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis |
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| MA Wen-fang,DENG Hui-lian,CAI Yi and YU Jiao.Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis[J].China Journal of Experimental Traditional Medical Formulae,2013,19(3):112-115. | |
| Authors: | MA Wen-fang DENG Hui-lian CAI Yi and YU Jiao |
| Institution: | Guangxi University of Chinese Medicine, Guangxi 530001 China;Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;Guangxi University of Chinese Medicine, Guangxi 530001 China;Guangxi University of Chinese Medicine, Guangxi 530001 China;Guangxi University of Chinese Medicine, Guangxi 530001 China;Maternal and Child Healthcare Hospital of Anji County in Zhejiang Province, Anji 313300, China |
| Abstract: | Objective:To establish a quantitative method for the determination of total flavonoids in Scoparia dulcis by UV-VIS spectrophotometry. Method: The effects of species, concentration, and dosage of chromogenic reagent were studied, and NaNO2-Al(NO3)3-NaOH colour reaction system was used for content determination of total flavonoids in Scoparia dulcis. Result: Using rutin as comparison sample, the detection wave length was set at 506 nm. There was good linear relationship during 4.8-96.2 mg·L-1 (r=0.999 3). The average recovery rate was 103.71% (RSD 2.68%, n=6). Conclusion: The method is simple, rapid, accurate and can be used for the quantitative method of S. dulcis. |
| Keywords: | Scoparia dulcis total flavonoids quantitative method colour reaction system optimization |
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