星点设计-效应面法优选凤仙透骨草的提取工艺

目的:采用星点设计-效应面法优化凤仙透骨草的提取工艺.方法:以乙醇体积分数、提取时间、溶媒比为自变量,凤仙透骨草总黄酮提取率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,采用效应面法优选工艺条件,并进行预测分析.结果:最佳提取工艺为加23.83倍量81.55％乙醇提取164.08 min,提取预测值与真实值偏差-1.08％,二项式拟合复相关系数0.987 1.结论:优化的凤仙透骨草提取工艺简便、精密度高、可预测性良好.

Optimization of Extraction Technology for Impatiens balsamina by Central Composite Design/Response Surface Methodology

Objective: To optimize extraction technology of Impatiens balsamina by central composite design/response surface methodology. Method: With ethanol concentration,extraction time and solvent ratio as independent variables,yield of total flavonoids from I. balsamina as dependent variable,levels of independent variables were regressed by multiple linear and fitted by binomial,response surface method was used to optimize extraction process,and prediction analysis was carried out by comparing observed and predicted values. Result: Optimal extraction technology was as following:extracted 164.08 min with 23.83 times the amount of 81.55% ethanol,deviation between observed and predicted values was-1.08%,multiple correlation coefficient of binomial fitting model was 0.987 1. Conclusion: This optimized extraction process of I. balsamina was simple with high precision and good predictability.