妇舒保凝胶中黄柏、苦参的提取工艺优选

目的:优选妇舒保凝胶中黄柏、苦参的提取工艺。方法:以出固率和盐酸小檗碱、苦参碱的得率为指标,单因素试验考察水煎煮法、乙醇回流法及乙醇渗漉法对黄柏、苦参提取效率的影响;选取乙醇体积分数、乙醇用量、提取次数、提取时间为考察因素,采用正交试验优选妇舒保凝胶中黄柏、苦参的提取工艺。结果:选择乙醇回流法进行提取,最佳提取工艺为加8倍量50%乙醇提取3次,每次1.5 h,总生物碱得率约2.47%。结论:该优选工艺稳定可行、重复性良好,为妇舒保凝胶中有效成分的提取提供试验依据。

Optimization of Extraction Technology of Phellodendrom chinense and Sophora flavescens from Fushubao Gel

Objective: To optimize extraction technology of Phellodendrom chinense and Sophora flavescens from Fushubao gel. Method: Taking yield of matrine,berberine hydrochloride and extract as indexes,single factor test was used to choose extraction method,including water boiling method,ethanol reflux method and ethanol percolation method;Orthogonal test was designed to optimize extraction technology of P. chinense and S. flavescens from Fushubao gel with extraction times,extraction time,the amount and concentration of ethanol as factors. Result: Ethanol reflux method was selected,optimum extraction technology was as follows:extracted 3 times with 8 times the amount of 50% ethanol,1.5 hours each time.Yield of total alkaloid was about 2.47%. Conclusion: This optimized process was stable,feasible and reproducible,it could provide experimental basis for extraction of active ingredients from Fushubao gel.