| 不同品种、不同产地溪黄草咖啡酸与迷迭香酸的含量测定 |
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| 引用本文： | 朱德全,黄松,陈建南,谭玉莲,屈莹莹,庄妍劼.不同品种、不同产地溪黄草咖啡酸与迷迭香酸的含量测定[J].中国实验方剂学杂志,2013,19(2):114-117. |
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| 作者姓名： | 朱德全 黄松 陈建南 谭玉莲 屈莹莹 庄妍劼 |
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| 作者单位： | 1. 广州中医药大学,广州,510006
2. 广州中医药大学,广州 510006;东莞广州中医药大学中医药数理工程研究院,广东东莞 523808 |
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| 摘 要： | 目的:测定不同品种、不同产地溪黄草咖啡酸与迷迭香酸的含量。方法:采用HPLC,Dikma Diamonsil(2)(C184.6 mm×250 mm,5μm)色谱柱,以乙腈-0.3%磷酸为流动相梯度洗脱,流速1.0 mL.min-1,柱温室温,检测波长329 nm。结果:咖啡酸和迷迭香酸的线性范围分别为0.045 4～0.908 0μg(r=0.999 6)和0.219 2～4.384 0μg(r=0.999 9),平均回收率(n=5)分别为97.33%(RSD 2.20%),103.32%(RSD 1.84%)。不同品种、不同产地溪黄草药材中咖啡酸、迷迭香酸的含量差异较大,其中纤花变种的溪黄草两者的平均含量最高。结论:该方法简单快捷,适合于溪黄草中酚酸成分的含量测定研究,为溪黄草的质量控制提供了依据。
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| 关 键 词： | 溪黄草 咖啡酸 迷迭香酸 HPLC |
| 收稿时间： | 8/4/2012 12:00:00 AM |
Determination of Caffeic Acid and Rosmarinic Acid in Rabdosia serra from Different Species and Different Habitat |
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| ZHU De-quan,HUANG Song,CHEN Jian-nan,TAN Yu-lian,QU Ying-ying and ZHUANG Yan-jie.Determination of Caffeic Acid and Rosmarinic Acid in Rabdosia serra from Different Species and Different Habitat[J].China Journal of Experimental Traditional Medical Formulae,2013,19(2):114-117. |
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| Authors: | ZHU De-quan HUANG Song CHEN Jian-nan TAN Yu-lian QU Ying-ying and ZHUANG Yan-jie |
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| Institution: | New Drug R&D Department in Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug R&D Department in Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Dongguan Institution for Mathematics and Theoretics Engineering Research, Dongguan 523808, China;New Drug R&D Department in Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Dongguan Institution for Mathematics and Theoretics Engineering Research, Dongguan 523808, China;New Drug R&D Department in Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug R&D Department in Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug R&D Department in Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | Rabdosia serra caffeic acid rosmarinic acid HPLC |
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