榅桲叶和果实中总黄酮提取工艺优选

目的:优选新疆榅椁叶与果实中总黄酮的提取工艺.方法:以总黄酮含量为指标,采用正交试验考察加醇量、超声提取时间、超声功率、乙醇体积分数4个因素对提取工艺的影响.结果:榅桲叶和果实中总黄酮最佳提取工艺均为A3B3C3D1,即加30倍量60％乙醇超声提取40 min,超声功率80％.叶中总黄酮含量较果实中高.结论:优选的总黄酮提取工艺稳定可行,为新疆医药的开发提供实验依据.

Optimization of Extraction Technology for Total Flavonoids from Leaves and Fruit of Cydonia oblonga

Objective: To optimize extraction technology of total flavonoids from leaves and fruit of Cydonia oblonga. Method: With the content of total flavonoids as index,orthogonal design was used to investigate influence of four factors on extraction technology,including the concentration of ethanol,ultrasonic time,ultrasonic power and ethanol amount. Result: Optimum extraction technologies of leaves and fruit were A₃B₃C₃D₁,ultrasonic extracted 40 min with 30 times the amount of 60% ethanol,ultrasonic power 80%,the content of total flavonoids in leaves was higher than fruit. Conclusion: This optimized technology was stable and feasible,and it could provide experimental basis for development of Chinese medicine in Xinjiang.