胃舒灵对慢性胃炎模型大鼠胃黏膜EGF和EGFR表达的影响

目的:观察胃舒灵(WSL)对慢性胃炎模型大鼠表皮生长因子(EGF)和表皮生长因子受体(EGFR)的影响,探讨其治疗慢性胃炎的作用机制.方法:将大鼠随机分成正常组(等容量蒸馏水)、模型组(等容量蒸馏水)、温胃舒颗粒组(3.60 g·kg-1)、胃舒灵低剂量组(0.56 g·kg-1),胃舒灵高剂量组(2.22 g·kg-1),后4组采用水杨酸钠复合法复制慢性胃炎模型.模型成功后用胃舒灵对实验性大鼠慢性胃炎进行治疗6周后,采用免疫组化法测定大鼠胃黏膜组织EGF和EGFR的变化.结果:胃舒灵(0.56,2.22 g·kg-1)能显著改善模型大鼠的症状,胃黏膜的病理及评分(P＜0.01),胃舒灵(0.56,2.22 g·kg-1)明显降低EGFR含量表达(P＜0.01),胃灵舒(0.56 g·kg-1)能降低EGF含量表达(P＜0.05).结论:胃舒灵具有良好的治疗慢性胃炎的作用,其机制与下调慢性胃炎大鼠胃黏膜表皮生长因子含量及表皮生长因子受体有关.

Effects of Weishuling on the Expression of Epidermal Growth Factor and Epidermal Growth Factor Receptor in Stomach Mucosa of Chronic Gastritis Rats

Objective: To observe effects of Weshuling(WSL)on the expression of epidermal growth factor (EGF)and epidermal growth factor receptor (EGFR)in the established rats model of chronic gastritis, so as to explore the therapeutic mechanism of WSL. Method: Mice were randomly divided into normal control group, model control group, Wenweishu granules group, high and low doses groups with WSL. Experimental gastritis were prepared in the later four groups by oral intake of sodium salicylate,drugs was administrated in the model control group,Wenweishu granules group, high and low doses groups with WSL for six weeks, EGF and EGFR from stomach mucosal organization of chronic gastritis model rats were measured by immunohis to chemical. Result: WSL(0.56,2.22 g·kg^-1) could significantly reduce reperfusion arrhythmias ratings rate (P<0.01) and the expression of EGFR(P<0.01). WSL(0.56 g·kg^-1) could reduce the expression of EGF(P<0.05). Conclusion: WSL decoction has protective effect on chronic gastritis. Its mechanism might be related to down-regulating the expressions of EGF and EGFR.