木香中去氢木香内酯和木香烃内酯提取工艺优选

目的:优选木香中去氢木香内酯和木香烃内酯的提取工艺。方法:以木香烃内酯和去氢木香内酯提取率为指标,采用单因素试验考察提取方法;以木香烃内酯和去氢木香内酯提取率为指标,选取乙醇体积分数、料液比、提取时间及提取次数为考察因素,通过正交试验优选2种内酯的提取工艺。结果:最佳提取工艺为采用乙醇温浸法,加6倍量90%乙醇于40℃温浸提取2次,每次2 h。木香烃内酯提取率92.3%,去氢木香内酯提取率94.1%,得膏率12.7%。结论:优选的提取工艺稳定可行,木香中2种内酯的提取率高,适用于工业大生产。

Optimization of Extracting Technology of Costunolide and Dehydrocostus Lactone from Aucklandia lappa

Objective: To optimize extracting technology of costunolide and dehydrocostus lactone from Aucklandia lappa. Method: With extraction rate of costunolide and dehydrocostus as indexes,extraction method was screened by single factor test;With extraction rate of costunolide and dehydrocostus as indexes,orthogonal test was used to evaluate effect of 4 factors on extracting technology,including solid-liquid ratio,the concentration of ethanol,extraction time and times. Result: The best extraction method was ethanol warm immersion method,optimum extracting technology was as follows:warm immersion extracted 2 times with 6 times the amount of 90% ethanol,respectively,extraction time of 2 hours.Extraction rate of costunolide and dehydrocostus lactone were 92.3,94.1%,respectively,and extract yield was12.7%. Conclusion: This optimized extracting technology was stable and feasible with high extraction rate of two kinds lactone from A. lappa,it was suitable for industrial production.