| HPLC测定苦豆子不同部位中苦豆碱的含量 |
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| 引用本文： | 魏蕾初,邓虹珠,张丽,刘振龙,易延逵. HPLC测定苦豆子不同部位中苦豆碱的含量[J]. 中国实验方剂学杂志, 2013, 19(4): 140-143 |
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| 作者姓名： | 魏蕾初 邓虹珠 张丽 刘振龙 易延逵 |
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| 作者单位： | 1. 南方医科大学中医药学院,广州,510515
2. 广东省食品药品检验所,广州,510180 |
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| 摘 要： | 目的:建立苦豆子中苦豆碱的HPLC法；对苦豆子药材不同部位中苦豆碱含量进行考察,确定药材最佳采收部位.方法:采用Kromasil NH2(4.6 mm×250 mm,5μm)色谱柱,以乙腈-无水乙醇-3％磷酸水溶液(70:15:15)为流动相,检测波长205 nm,柱温25℃,流速1.0 mL· min-1.结果:苦豆碱的线性范围为11.21 ～224.20 mg·L-1(r=0.9999),平均回收率(n =6)98.18％ (RSD 4.1％)；苦豆子叶、茎中苦豆碱含量分别为0.38％,0.02％,豆荚、花及种子中不含苦豆碱.结论:该方法简便、准确、重复性好,可以作为苦豆子中苦豆碱的含量测定方法；研究结果显示苦豆碱在叶子中含量最高,可以将叶子作为提取分离苦豆碱的最佳药材部位.
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| 关 键 词： | 苦豆子 苦豆碱 高效液相色谱法 药用部位 含量测定 |
| 收稿时间： | 2012-09-19 |
Determination of Aloperine in Different Parts of Sophora alopcuroides by HPLC |
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| WEI Lei-chu,DENG Hong-zhu,ZHANG Li,LIU Zhen-long and YI Yan-kui. Determination of Aloperine in Different Parts of Sophora alopcuroides by HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(4): 140-143 |
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| Authors: | WEI Lei-chu DENG Hong-zhu ZHANG Li LIU Zhen-long YI Yan-kui |
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| Affiliation: | School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China;School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China;School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China;Guangdong Institude for Food and Drug Control, Guangzhou 510180, China;School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China |
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| Abstract: | |
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| Keywords: | Sophora alopcuroides aloperine HPLC medicinal part determination |
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