补骨脂、生地黄对正常大鼠体温及ATP酶活性的影响

目的:探讨补骨脂、生地黄对正常大鼠体温及ATP酶活性的影响.方法:将大鼠随机分为空白对照组、附子热性对照组、大黄寒性对照组、补骨脂组与生地黄组,附子组6.75 g·kg-1,大黄组13.5 g·kg-1,补骨脂组4.05 g·kg-1,生地黄组6.75 g·kg-1,按10 mL·kg-1ig,1次/d,每周给药6d,停药1d,共给药28 d,空白组ig等量(10 mL·kg-1)生理盐水.给药28 d后测量大鼠趾温、肛温,大鼠处死后,取出肝脏,制备组织匀浆,测定三磷酸腺苷(ATP)酶活性.结果:与空白对照组相比,补骨脂组大鼠趾温、肛温均明显升高(P＜0.05),生地黄组大鼠趾温、肛温均明显降低(P＜0.05).与空白对照组相比,补骨脂组肝组织中Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性均明显升高(P＜0.05)；生地黄组Na+-K+-ATP酶,Ca2+-Mg2+-ATP酶活性均明显降低(P＜0.05).结论:补骨脂、生地黄对正常大鼠体温及ATP酶活性影响明显,提示寒、热药性不同的药物与对体内ATP酶活性的影响不同可能是对体温产生不同的作用趋势的原因之一.

Effect of Psoraleae Fructus and Rehmanniae Radix on Body Temperature and ATPase Activity in Rats

Objective:To discuss the effect of Psoraleae Fructus and Rehmanniae Radix on body temperature and ATPase activity in rats. Method: Fifty rats were randomly divided into the blank control group, Aconiti Lateralis Radix Praeparata control group, Rhei Radix et Rhizoma control group, psoraleae fructus group and Rehmanniae Radix group. the dosage of aconiti Lateralis Radix Praeparata control group was 6.75 g·kg^-1; the dosage of Rhei Radix et Rhfizoma control group was 13.5 g·kg^-1; the dosage of Psoraleae Fructus group was 4.05 g·kg^-1; the dosage of Rehmanniae Radix group was 6.75 g·kg^-1. The rats were fed with the amount of 10 mL·kg^-1 for 28 days (the blank group was fed with equal volume of physiological saline irrigation), 1 time a day, lasting 6 days, stopping 1 day. The toe temperature and Rectal Temperature of the rats were measured 28 days after administration. The liver was removed and made into homogenate after the rats were put to death. The adenosine triphosphate(ATP) ase activity was measured. Result: The toe temperature and rectal temperature of psoraleae fructus group were obviously higher than those of blank control group (P<0.05). The toe temperature and rectal temperature of rehmanniae radix group were obviously lower than those of blank control group (P<0.05). The activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in liver tissue in psoraleae fructus group were obviously higher than those in blank control group (P<0.05). The activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in rehmanniae radix group were obviously lower than those in blank control group (P<0.05). Conclusion: Psoraleae fructus and rehmanniae radix have a significant influence on body temperature and ATPase activity. It is pointed that the different influences on ATPase activity of herbs with different properties might be one of the reasons why herbs with different properties could made different trends on body temperature. Its specific mechanisms should be studied further.