当归补血-活血药对功效取向研究

目的:研究当归补血-活血药对(当归-熟地黄、当归-黄芪、当归-白芍、当归-赤芍、当归-川芎、当归-桃仁)功效取向。方法:ICR小鼠,雌雄各半,随机分为正常组、模型组、当归-熟地黄、当归-黄芪、当归-白芍、当归-赤芍、当归-川芎和当归-桃仁药对组(10.4 g.kg-1),正常组和模型组ig等容积生理盐水,连续8 d。以乙酰苯肼与环磷酰胺合用建立血虚小鼠模型,模型组和给药组于给药开始第1天ip环磷酰胺200 mg.kg-1,第6天sc乙酰苯肼100 mg.kg-1。第8天测定外周血象、胸腺指数、脾脏指数和红细胞膜ATP酶活力,比较当归药对补血功效;SD大鼠,雌雄各半,分组同上(7.2 g.kg-1),正常组和模型组ig等容积生理盐水,连续7 d。以肾上腺素加冰水浴刺激建立急性血瘀大鼠模型,模型组和给药组于第6天sc肾上腺素(0.8mg.kg-1),间隔4 h,共2次,两次sc肾上腺素之间,将大鼠浸入冰水内4 min。第7天测定血液流变学和凝血四项指标,比较当归药对活血功效。结果:与模型组相比,当归-熟地黄、当归-黄芪和当归-白芍药对能显著升高血红蛋白含量(P<0.01)和红细胞压积(P<0.01或P<0.05),降低其脾脏指数(P<0.01),综合补血作用更强;与模型组相比,当归-熟地黄、当归-赤芍、当归-川芎和当归-桃仁药对能显著降低全血低切还原黏度、血沉方程K值和红细胞聚集指数(P<0.01或P<0.05),显著延长活化部分凝血活酶时间(P<0.01),综合活血作用更强。结论:当归-黄芪、当归-白芍药对偏于补血,当归-赤芍、当归-川芎和当归-桃仁药对偏于活血,当归-熟地黄药对补血、活血作用均较强。

Characteristics of Blood Nourishing and Blood Circulation Activating Efficacy Preference of Angelicae Sinensis Radix Herb Pairs

Objective: To study the characteristics of blood nourishing and blood circulation activating efficacy preference of the Angelicae Sinensis Radix herb pairs. Method: ICR mice, half male and half female, were randomly divided into control group,model group,Angelicae Sinensis Radix - Rehmanniae Radix Preparata (Danggui-Shudihuang), Angelicae Sinensis Radix-Astragali Radix (Danggui-Huangqi), Angelicae Sinensis Radix-Paeoniae Radix Alba (Danggui-Baishao), Angelicae Sinensis Radix-Paeoniae Rubra Radix (Danggui-Chishao), Angelicae Sinensis Radix-Chuanxiong Rhizoma(Danggui-Chuangxiong) and Angelicae Sinensis Radix\|Persicae Semen (Danggui-Taoren) groups (10.4 g·kg^-1). Control and model groups were ig given the same volume physiological saline for 8 days. Acetylphenyhydrazine and cyclophosphamide were used to copy the blood-deficiency model mice by ip cyclophosphamide (200 mg·kg^-1) at the first day and sc acetylphenyhydrazine (100 mg·kg^-1) at the sixth day. Periphera hemogram, organ index and BRC ATPase activity were detected at the 8th day to compare the blood nourishing efficacy. SD rats, half male and half female, the same group as above (7.2 g·kg^-1), control and model groups were ig given the same volume physiological saline for 7 days. Epinephrine hydrochlaride and ice-water stimulation were used to copy acute blood stasis model rats by sc acetylphenyhydrazine (0.8 mg·kg^-1) at the 6th day(interval of 4 h, 2 times) and ice-water stimulation for 4 min. Aemorheology, blood clotting tetrachoric index were detected at the 7th day to compare the blood circulation activating efficacy. Result: Compared with model group, Danggui-Baishao, Danggui-Huangqi and Danggui-Shudihuang could increase HGB(P<0.01), HCT (P<0.01 or P<0.05) and reduced SI (P<0.01) and had better blood nourishing effect. Danggui-Shudi, Danggui-Chuanxiong, Danggui-Chishao and Danggui-Taoren could reduce whole blood low reduction viscocity, bood sedimentation equation K, and AI (P<0.01 or P<0.05) and lengthen APTT (P<0.01) and had better blood activating effect. Conclusion: Danggui-Baishao and Danggui-Huangqi herb pairs have better blood nutriting efficacy. Danggui-Chuanxiong, Danggui-Chishao and Danggui-Taoren herb pairs have better blood circulation activating efficacy. Danggui-Shudihuang herb pairs have well nutriting and circulation activating efficacy at the same time.