壳聚糖絮凝沉降法对蒽醌类成分的影响

目的:研究壳聚糖絮凝沉降法对蒽醌类成分的影响。方法:选择大黄为研究对象,采用壳聚糖沉降法处理大黄水提液,以大黄酸、大黄素、芦荟大黄素、大黄酚、大黄素甲醚质量分数和浸膏率为指标,选取药液质量浓度、壳聚糖加入量、药液pH为考察因素,通过正交试验优选壳聚糖絮凝沉降工艺;并与乙醇沉淀法比较。结果:优选的壳聚糖絮凝沉降工艺为药液质量浓度0.1 g.mL-1,壳聚糖加入量10%,药液pH 6。壳聚糖沉淀法和乙醇沉淀法均能较好地使水提液沉淀,但两者均会造成蒽醌类成分的损失,前者在降低浸膏率、保留有效成分方面优于后者。2种沉淀法纯化后,药液中蒽醌类成分与未纯化药液比较无显著性差异。结论:壳聚糖絮凝沉降法对蒽醌类成分影响较乙醇沉淀法小,可用于含蒽醌类成分中药的沉淀,但是否适合于大生产有待进一步研究。

Effect of Chitosan Flocculation Sedimentation Method on Anthraquinones

Objective: To study on effect of chitosan flocculation sedimentation method on anthraquinones. Method: Taking Rheum palmatum as research object,water extracting liquid of R. palmatum was handled by chitosan sedimentation,with the mass fraction of rhein,emodin,aloe-emodin,chrysophanol,physcion and extract rate as indexes,orthogonal test was used to optimize chitosan flocculation sedimentation by taking the mass concentration and pH of water extracting liquid,the amount of chitosan as factors;And compared with ethanol precipitation method. Result: Optimized chitosan flocculation sedimentation technology was:the mass concentration of water extracting liquid 0.1 g· mL^-1 with pH 6,the amount of chitosan 10%.Both chitosan flocculation sedimentation method and ethanol precipitation method could precipitate water extract preferably,but also lead to loss of anthraquinones.The former was better than the latter in reducing extract rate and retaining active ingredients.There was no significant difference between the purified and unpurified liquid by these two kinds of precipitation methods. Conclusion: Effect of anthraquinones by chitosan flocculation sedimentation method was lower than ethanol precipitation method,it could be used in precipitation of Chinese materia medica containing anthraquinones,but it needed more research to decide if this method fitted expandable production.