三七总皂苷保护PC12细胞对抗过氧化氢损伤的作用

目的:考察三七总皂苷在PC12细胞中对过氧化氢引起损伤的细胞保护作用.方法:采用MTT法考察0.01～100mg·L-1三七总皂苷对正常PC12细胞活力的影响,并在H2O2刺激的PC12细胞中考察0.1～10 mg·L-1的三七总皂苷对细胞活力的影响,通过对细胞活力的考察选择三七总皂苷合适的实验剂量,测定三七总皂苷0.1,1 mg·L-1对H2O2刺激的PC12细胞中活性氧水平、过氧化氢酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力的影响以及对丙二醛(MDA)含量的影响.结果:三七总皂苷在0.1～10 mg·L-1浓度能提高正常PC12细胞活力,并能显著提高H202刺激的PC12细胞活力(P＜0.01).0.1,1mg·L-12个剂量的三七总皂苷均能显著减少H2O2刺激的PC12细胞中活性氧水平,提高SOD活力(P ＜0.001)和GSH-Px活力(P ＜0.001),并减少MDA生成(P ＜0.001).结论:三七总皂苷能通过增强细胞内抗氧化酶活力,保护PC12细胞对抗氧化损伤.

Effect of Panax notoginseng Saponins Protect PC12 Cell Against Hydrogen Peroxide Induced Damage

Objective: To investigate the protective effect of Panax notoginseng saponins (PNS) on hydrogen peroxide (H₂O₂) induced oxidative damage in PC12 cell. Method: MTT method was employed to determine the viability in PNS (0.01-100 mg·L^-1) treated PC12 cell and PNS (0.1-10 mg·L^-1) plus H₂O₂ treated PC12 cell. Thereafter, intracellular reactive oxygen species amounts, activity of SOD, GSH-Px, as well as MDA concentration were determined at optimal dosage of PNS selected based on MTT experiment. Result: PC12 cell viability was increased by PNS at 0.1-10 mg·L^-1, and the viability of H₂O₂ treated PC12 cell was increased by PNS at 0.1-10 mg·L^-1. Furthermore, PNS at 0.1-1.0 mg·L^-1 significantly decreased intracellular reactive oxygen species levels, as well as enhanced SOD activity (P<0.001) and GSH-Px activity (P<0.001). But MDA concentration was decreased significantly (P<0.05). Conclusion: PNS can protect PC12 cell against H₂O₂ induced damage via enhancement of intracellular antioxidant enzyme activity.