不同产地雄黄及其炮制品中二硫化二砷和可溶性砷含量比较

目的:比较研究不同产地雄黄及其炮制品中主成分As2S2及可溶性砷盐As2O3的含量差异。方法:采用2010年版《中国药典》雄黄项下的滴定法测定不同产地雄黄及其炮制品中As2S2的含量,采用AFS-230E型原子荧光光度计检测上述样品中可溶性砷盐As2O3含量。结果:不同产地的雄黄及其炮制品中As2S2,As2O3含量存在显著差异。雄黄炮制后,炮制品中As2S2含量均提高,As2O3含量均明显降低。除吉林和江苏2个产地的的雄黄及其炮制品中As2S2含量<90.0%,其余样品均符合药典规定;且雄黄炮制品中As2O3含量均能控制在1.7 mg.g-1以下。结论:研究结果可为指导雄黄临床合理用药提供参考依据。

Comparative Study on Contents of As2S2 and As2O3 in Realgar and Processed Products

Objective: To study the content difference of the principal component (As₂S₂) and the soluble arsenic salt (As₂O₃) in realgar and it processed products from different origins. Method: As₂S₂ content was determined by titration based on the analysis method of realgar recorded in Chinese Pharmacopoeia (2010 edition),and As₂O₃ content was detected using AFS-230E Atomic fluorescence spectrophotometer. Result: The contents of As₂S₂ and As₂O₃ were significantly different in realgar and its processed products from different origins. After processing, As₂S₂ content in the processed products was obviously increased, while As₂O₃ content was significantly reduced. Except that As₂S₂ content in realgar and its processed products from Jilin province and Jiangsu province was less than 90.0%, the rest of the samples can meet the pharmacopoeia requirements, and As₂O₃ content in all of the processed products can be controlled below 1.7 mg· g^-1. Conclusion: This findings can provide a reference for guiding rational clinical applications of realgar.