冷缺血与热缺血对大鼠肝细胞凋亡的影响

目的:观察冷保存与热缺血对大鼠肝细胞凋亡的影响。方法:用流式细胞检测技术，分组检测冷保存时和热缺血时的肝细胞凋亡率与增殖率。结果:冷保存时各组之间的肝细胞凋亡率与增殖率差异无显著性（P>0.05）；随着热缺血时间的延长，细胞凋亡率明显加重（P＜0.05），但细胞增殖率的改变不明显（P>0.05）。结论:大鼠肝脏冷保存/再灌注时肝细胞凋亡可能在有氧再灌注后加重；大鼠肝脏热缺血/再灌注时肝细胞凋亡可能在缺血时和再灌注后均可加重。

The impact of cold preservation and warm ischemia on hepatocytes apoptosis in rats

Abstract:Objective:To observe the effect of cold preservation and warm ischemia on apoptosis of hepatocytes in rats.Methods :The hepatocyte apoptosis rate and regenerative rate of cold preservation group and warm ischemia group were detected and compared through flow cytometry.Results:There was no difference in the hepatocyte apoptosis rate and regenerative rate(P＜0.05) at different time points during cold preservation. The hepatocyte apoptosis rate increased as the warm ischemia with time incresed (P＜0.05); whereas there was no difference in regenerative rate(P>0.05).Conclusions:The hepatocyte apoptosis of rats is more severe after aerobic reperfusion during cold preservation /reperfusion. It is more severe after ischemia and reperfusion during warm ischemia/reperfusion periods.