Diagnosis of type III hyperlipoproteinemia: contribution of an air-driven ultracentrifuge associated with an original tube-slicer (author's transl]

Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three hours (Airfuge Beckman instruments). An original tube-slicer allows the separation of the d less than 1.006 lipoproteins located into top fractions. This simple micromethod might confirm diagnosis of type III in patients and would be useful for clinical laboratories.