Pycnogenol Cytotoxicity in Pancreatic INS‐1E β cells Induced by Calcium Dysregulation |
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Authors: | Jana Viskupicova Petronela Zizkova Lucia Rackova Lubica Horakova |
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Institution: | 1. Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia;2. Department of Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovakia |
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Abstract: | Natural standardized flavonoid extract from the bark of Pinus pinaster, Pycnogenol (Pyc), was recently found to decrease intensively the activity of sarcoplasmic reticulum Ca2+‐ATPase of rabbit skeletal muscle (SERCA1). On the basis of this inhibitory effect in a cell‐free system and similarities of SERCA1 to its other isoforms, proapoptotic properties of Pyc may be expected in cellular systems. Pycnogenol (40–100 μg/mL) induced a concentration‐dependent decrease of the viability of pancreatic INS‐1E β cells associated with induction of apoptosis. In addition, intracellular Ca2+ level increase was found along with reduction of protein expression level of SERCA2b and impairment of insulin secretion by β cells. These facts indicate that Pyc may induce apoptosis by impairment of calcium homeostasis. Copyright © 2017 John Wiley & Sons, Ltd. |
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Keywords: | calcium homeostasis flavonoid extract pro‐apoptotic properties insulin secretion |
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