淋巴细胞中NF-κB p65活性水平在Fx1A诱导的口服耐受大鼠中的变化

目的：探讨核因子-κB(NF-κB)p65在口服耐受发生中的活性改变及其意义。方法：雌性Wistar大鼠30只随机分成两组：①口服耐受组，用小剂量(每次8mg／2mL PBS)的Fx1A抗原灌胃。②对照组，用PBS2mL／次灌胃。观察大鼠迟发型超敏反应(DTH)，进行脾淋巴细胞增殖试验，了解大鼠免疫功能的改变。以免疫组化和ELISA法，检测大鼠淋巴组织中NF-κB p65的活性及TGF-β1的表达。结果：与对照组相比较，口服耐受组大鼠DTH和抗原特异性淋巴细胞增殖反应明显受抑制；肠黏膜Peyer’s淋巴结(PP结)中NF-κB p6S的活性及TGF-β1的表达明显增加。脾淋巴组织中NF-κB p65的活性降低，但TGF-B1的表达明显增加。结论：低剂量Fx1A抗原诱导的口服耐受的发生，可能与不同部位免疫细胞内NF-κB p65的活性改变有关。

Change of activity of NF-κB p65 in lymphocytes from rat with oral tolerance induced by Fx1A

AIM: To explore the activity change and significance of NF-kappaB p65 in lymphocytes from rats with oral tolerance induced by Fx1A. METHODS: 30 female Wistar rats were divided into two groups (oral tolerance and control groups), which stomachs were perfused with Fx1A and PBS, respectively. The delayed-type hypersensitivity (DTH) was evaluated by skin test, the immune function was detected by splenic lymphocyte proliferation test, the activity of NF-kappaB p65 and expression of TGF-beta1 in lymphoid tissues were examined by immunohistochemical staining and Sandwich ELISA. RESULTS: In comparison with control group, the DTH and antigen-specific lymphocyte proliferation reaction in oral tolerance group were suppressed significantly. The activity of NF-kappaB p65 and expression of TGF-beta1 in Peyer's patch were increased notably. However, the activity of NF-kappaB p65 was decreased, but expression of TGF-beta1 was increased markedly in splenic lymphocytes. CONCLUSION: Information of oral tolerance induced by Fx1A may be related to the change of NF-kappaB p65 activity in lymphocytes of body's different places.