人胚神经干细胞的冻存与复苏

目的：研究神经干细胞浆存复苏后的成活率及再培养生长情况。方法：利用无血清培养基短期体外培养胚胎来源的神经干细胞后，以冻存液（体积分数为70％DMEM／F12，体积分数为20％胎牛血清，10％DMSO）进行冻存，于1周，4周，8周，12周，16周，20周，24周分别复苏，计活细胞比例并进行再培养。结果：复苏后神经干细胞的成活比例分别为68％、65％、65％、62％、61％、60％、60％，再培养生长良好。结论：本实验对神经干细胞成功地进行了冻存，复苏和再培养，对临床择期进行神经干细胞移植手术和建立“神经干细胞库”具有重要的意义。

Cryopreservation and thawing of human embryonic neural stem cells

Aim: To study the suruiual rate of neural stem cells and its growth after cryopreservation and thawing. Methods: The neural stem cells originated from human embryos were cultivated with serum-free medium in vitro for a short time and cryopreserved in liquid nitrogen. The cryopreserved cells were resuscitated at 1 week,4 week,8 week, 12 week, 16 week,20 week and 24 week respectively. The suruiual rate of cells were calculated and the cells were incubated again in serum-free medium. Result:The suruiual rates of neural stem cells were 68% ,65% ,65% ,62% ,61% ,60% , and 60% respectively, and the cells grew very well after thawing. Conclusion;The neural stem cells were successfully cryopreserved, resuscitated,and re-cultured, which would have great importance to the selected-date operations of neural stem cell transplantation and the establishment of neural stem cell depository.