| 实验性感染斯氏狸殖吸虫大鼠血清抗体三种检测方法的比较 |
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| 引用本文： | 朱名胜,罗冬青,宋明华,王绍基.实验性感染斯氏狸殖吸虫大鼠血清抗体三种检测方法的比较[J].山西医科大学学报,2008,39(2):145-146. |
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| 作者姓名： | 朱名胜 罗冬青 宋明华 王绍基 |
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| 作者单位： | 1. 郧阳医学院寄生虫学教研室,十堰,442000
2. 郧阳医学院药检学院 |
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| 摘 要： | 目的 比较三种血清学方法 对斯氏狸殖吸虫实验感染大鼠抗体检测的效果. 方法 采用斑点ELISA法(Dot-ELISA)、免疫酶染色试验(IEST)和后尾蚴膜试验检测斯氏肺吸虫病大鼠血清IgG抗体. 结果 三种方法 的阳性率分别为97.7%, 、97.5%和95.2%,差异无统计学意义(P＞0.05).用此三种血清学方法 检测正常大鼠、旋毛虫病大鼠、血吸虫病兔和蛔虫病人血清IgG抗体,除1例蛔虫病人血清IEST出现阳性反应外均为阴性反应. 结论 三种方法 对斯氏狸殖吸虫特异性IgG抗体的检测均有较好的敏感性和特异性.
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| 关 键 词： | 斯氏狸殖吸虫 抗体 血清学试验 实验性 感染 斯氏狸殖吸虫 正常大鼠 血清抗体 检测方法 比较 infected rats antibody detection methods 敏感性和特异性 阳性反应 阴性 人血清 蛔虫病 血吸虫病 旋毛虫病 统计学意义 |
| 文章编号： | 1007-6611（2008）02-0145-02 |
| 收稿时间： | 2007-09-17 |
| 修稿时间： | 2007年9月17日 |
Comparison of three serodiagnostic methods in detection of antibody in rats infected with Pagumogonimus skrjabini |
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| ZHU Ming-sheng,LUO Dong-qing,SONG Ming-hua,WANG Shao-ji.Comparison of three serodiagnostic methods in detection of antibody in rats infected with Pagumogonimus skrjabini[J].Journal of Shanxi Medical University,2008,39(2):145-146. |
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| Authors: | ZHU Ming-sheng LUO Dong-qing SONG Ming-hua WANG Shao-ji |
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| Abstract: | Objective To investigate the serum antibody in rats infected with Pagumogonimus skrjabini.MethodsPagumogonimus skrjabini antibodies in sera of infected rats were detected by Dot-ELISA,immunoenzymatic staining technique(IEST) and metacercaria membrane test.ResultsPositive detection rates of Dot-ELISA,IEST and metacercaria membrane test were97.7%,97.5% and 95.2%,respectively(P>0.05).Detecting healthy rats,rats with trichiniasis,rabbits with schistosomiasis,and ascariasis patients with three serodiagnistic methods,only one was positive with IEST in the patients of ascariasis,and other sera were all negative.ConclusionThe results show that these three methods have good sensitivity and specificity in detection of Pagumogonimus skrjabini antibodies. |
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| Keywords: | Pagumogonimus skrjabini antibodies serologic tests |
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