苏木精对人类膀胱癌T24细胞的杀伤及诱导凋亡作用

目的观察苏木精对人类膀胱癌T24细胞的杀伤及诱导凋亡的作用，探讨其对靶细胞杀伤的作用机制。方法将靶细胞培养于含药量分别为0、12．5、25、50、100、200μg／ml的RPMI1640培养基中，培养24h，倒置显微镜下观察细胞的形态学变化，收集靶细胞，采用锥虫蓝拒染法测定药物对靶细胞的杀伤作用。采用流式细胞术（FCM）检测不同药物质量浓度对靶细胞凋亡的影响。结果随着药物质量浓度的逐步增加，细胞发生形态学变化，且细胞死亡率逐步增加，对照组细胞死亡率为（2．63±0．29）％，各组细胞死亡率分别为（10．00±4．82）％、（21．88±3．42）％、（76．41±4．82）％、（92．27±7．62）％和（96．34±8．78）％。对照组细胞凋亡的自然发生率为0．47％；含药量为50μg／ml时，细胞凋亡率显著增高，达到43．18％，死细胞率为48．47％，活细胞率为8.35％；随着药物质量浓度的增大，细胞凋亡率逐渐降低。而死细胞率则逐渐上升。结论苏木精可通过诱导细胞凋亡和直接杀伤作用于靶细胞，在较低浓度下，可诱导靶细胞发生凋亡，药物浓度超过100μg／ml时，则能直接杀死靶细胞。

Hematoxylin's cytocidal and apoptosis-inducing effects on human urinary bladder cancer cell-T24

Objective To observe hematoxylin's cytocidal and apoptosis-inducing effects on human urinary cancer cell-T24,and its cytocidal mechanism to the target cell.Methods Target cells were incubated in the medium 1640 for 24h,which contained hematoxylin in dosage of zero(blank),12.5,25,50,100,200μg/ml,respectively;under inverted microscopy to observe target cells'morphologic change,and then harvest them;by trypan blue tmpochrome method to determine hematoxylin's cytocidal activity to the target cells;by flow cytomelry to detect the effects of hematoxylin in its different levels on target cells'apoptosis.Results The control group(without hematoxylin)showed their target cells in a fusiform adherent growth,plump,close-arranged,and with a good transparence.With the addition and increment of hematoxylin,target cells turned round,not adherent,pyknotic,with a bad transparence,as well as chromatin condensation,the cells clumped.Cell death rate of control group was(2.63±0.29)%,with the increased dosage of hematoxylin the cell death rate of test groups was(10.00±4.82)%,(21.88±3.42)%,(76.41±4.82)%,(92.27±6.54)%,and(96.34±8.70)%respectively.Flow cytometry showed cell apoptosis rate in control group was 0.47%(occurred spontaneously),but hematoxylin in dose of 50μg/ml made the apoptosis rate increased markedly,to 43.1 8%,dead cell rate 48.47%,and survival cell rate 8.35%.With the increased hematoxylin dose,cell apoptosis rate decreased gradually,while dead cell rate increased.Conclusion Hematoxylin can inhibit the target cell by two routes:to induce apoptose or kill it.In a lower dose it is able to induce target cell to apeptose;hematoxylin in a dose over 100μg/ml can directly kill the target cell.Making this trial for checking the cell's morphologic changes benefits determining the optimal dosage level and optimal acting duration for the apoptosis induction.