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| 根侧角化囊肿的CK18及基因表达的研究 | |
| 引用本文： | 鲁大鹏,刘思思,邢汝东,张敏,立本行宏.根侧角化囊肿的CK18及基因表达的研究[J].口腔颌面外科杂志,2010,20(1):33-36. |
| 作者姓名： | 鲁大鹏 刘思思 邢汝东 张敏 立本行宏 |
| 作者单位： | 1. 首都医科大学口腔医学院口腔细胞生物学实验室 2. 口腔颌面外科,北京,100050 3. 日本高知大学医学部齿科口腔外科,日本高知县南国市,783 |
| 基金项目： | 北京市留学人员科技活动择优资助市优秀项目;;北京市卫生局留学回国人员科研专项经费项目;;教育部留学回国人员科研启动基金(教外司留[2004]176号) |
| 摘 要： | 目的:对根侧角化囊肿与其它角化囊肿衬里上皮细胞角蛋白18(CK18)基因表达是否一致进行探讨。方法:1例根侧角化囊肿、2例颌骨牙源性角化囊肿和1例含牙囊肿的衬里上皮,使用CK18基因探针进行原位杂交,检测CK18 mRNA在衬里上皮细胞层的原位表达;同时进行CK18、PCNA、p53、p21、Bcl-2、Fas/Fas-L、Bax(B-9)、WAF-1、CEA的单克隆抗体免疫组织化学染色。结果:原位杂交法显示CK18 mRNA在根侧角化囊肿的表达为强阳性,其它角化囊肿衬里上皮表现为弱阳性;免疫组化染色结果显示PCNA和Bcl-2在根侧角化囊肿中表达为强阳性,在2例颌骨牙源性角化囊肿表达为弱阳性,在含牙囊肿中有表达;而p53、p21、Fas/Fas-L、CEA在病例中均为阴性表达;Bax(B-9)、WAF-1在1例角化囊肿中表达为弱阳性,在其余3例中均为阴性表达。结论:根侧角化囊肿与其他部位角化囊肿相比较,强阳性表达反映增殖能力的相关蛋白及阴性表达与凋亡有关蛋白、抑癌基因,提示根侧角化囊肿可能比其它部位角化囊肿增殖或复发的潜力更高。 |
| 关 键 词： | 根侧囊肿 细胞角蛋白18 角化囊肿 原位杂交 |
Cytokeratin 18(CK18)and its Gene Expression in Lateral Periodontal Cyst(Keratocyst) |
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| LU Da-peng,LIU Si-si,XING Ru-dong,ZHANG Min,Yukihiro Tatemoto.Cytokeratin 18(CK18)and its Gene Expression in Lateral Periodontal Cyst(Keratocyst)[J].Chinese Journal of Oral and Maxillofacial Surgery,2010,20(1):33-36. | |
| Authors: | LU Da-peng LIU Si-si XING Ru-dong ZHANG Min Yukihiro Tatemoto |
| Institution: | 1.Laboratory of Cell Biology/a>;2.Department of Oral and Maxillofacial Surgery/a>;School of Stomatology/a>;Capital Medical University/a>;Beijing 100050/a>;China/a>;3.Department of Oral and Maxillofacial Surgery/a>;School of Medicine/a>;Kochi University/a>;Kochi City 783/a>;Japan |
| Abstract: | Objective:To study the cytokeratin 18(CK18) and its gene in a lateral periodontal keratocyst and made comparison with odontogenic keratocysts in other locations.Methods:The epithelial linings of one lateral periodontal keratocyst,two mandibular odontogenic keratocysts and one dentigerous cyst were subjected to in situ hybridization for CK18 mRNA.At the same time,monoclonal antibody immunohistochemical staining for CK18,PCNA,p53,p21,Bcl-2,Fas/Fas-L,Bax(B-9),WAF-1 and CEA were processed.Results:In situ hybrid... |
| Keywords: | lateral periodontal cyst cytokeratin 18 keratocyst in situ hybridization |
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