AmpliSensor—聚合酶链反应定量检测肺结核患者外周血结 …

目的 探讨ＡｍｐｌｉＳｅｎｓｏｒ－聚合酶链反应定量检测外周血中结核分支杆菌ＤＮＡ在肺结核的应用价值。方法 采用ＱｌＡａｍｐ和ＡｃｕＰｕｒｅ法提取，制备全血中模板ＴＢ－ＤＮＡ，应用ＡｍｐｌｉＳｅｎｓｏｒ－ＰＣＲ定量检测，并与ＩＳ６１１０－单管巢式聚合酶链反应（ＳＮ－ＰＣＲ）作比较。结果２００例肺结核患者的血液标本中，两种方法测得结核分支杆菌ＤＮＡ的阳性率分别为６０．５％、６３．５％。８５例非结核肺病

Quantitative detection of Mycobacterium tuberculosis DNA in peripheral blood from patients with pulmonary tuberculosis by AmpliSensor PCR technique

Objective To evaluate the clinical value of the quantitative detection of Mycobacterium tuberculosis DNA by Amplisensor PCR in peripheral blood from patients with pulmonary tuberculosis Methods The model of Mycobacterium tuberculosis DNA in blood was made by QIAamp and AcuPure methods, quantitative detection of Mycobacterium tuberculosis DNA by AmpliSensor PCR technique and the results were compared with IS6110 single tube nested PCR (SN PCR) Results The peripheral blood from 200 patients with pulmonary tuberculosis were detected respectively by using AmpliSensor PCR and IS6110 SN PCR methods The positive rates of Mycobacterium tuberculosis DNA were 60 5% and 63 5% respectively, and that from 85 patients with non pulmonary tubeculosis were 4 7%, 8 2% Conclusions AmpliSensor PCR method showed higher sensitivity and specificity in detecting the Mycobacterium tubeculosis DNA from peripheral blood The variation of the TB DNA may be useful in evaluating the prognosis of the patients