Effects of adenosine on guinea pig pulmonary eosinophils |
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Authors: | Blair A. M. Walker |
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Affiliation: | (1) U.B.C. Pulmonary Research Laboratory, University of British Columbia St. Paul's Hospital, 1081 Burrard St, V6Z 1Y6 Vancouver, British Columbia, Canada;(2) Department of Pathology and Laboratory Medicine, Vancouver Hospital and Health Sciences Centre, Vancouver, B.C. |
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Abstract: | Extracellular adenosine has pharmacological activity on a wide variety of cell types and may play an important role as an inflammatory modulator with both pro- and anti-inflammatory activities. These studies examine the effects of adenosine on guinea pig pulmonary eosinophils. Adenosine alone did not directly induce superoxide (O2–) production. Pretreatment with adenosine primed the O2– response of guinea pig pulmonary eosinophils following the addition of 1 or 10M plateletactivating factor (PAF). Priming was seen at adenosine concentrations greater than 1 M and was maximal at 100M. At this maximal dose, adenosine priming increased the O2– response to 1M and 10M PAF by 86% and 51%, respectively. Priming by adenosine was not seen when ionomycin or phorbol myristate acid (PMA) were used as agonists. In fura-2 loaded eosinophils, the addition of 100 M adenosine resulted in a small but significant rise in intracellular calcium of 54.4 ±9.2 nM above baseline. In contrast, similar adenosine concentrations had no effect on cytosolic calcium levels in guinea pig neutrophils. These data demonstrate a pro-inflammatory role for adenosine in elicited guinea pig pulmonary eosinophils. |
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