粥样硬化斑块中组织因子和组织因子途径抑制物的表达

目的 观察股动脉粥样硬化斑块中组织因子TF和组织因子途径抑制物TFPI的表达、分布.方法 采用免疫组化、双染组化方法检测股动脉粥样硬化斑块中TF和TFPI的表达和分布,RT-PCR检测TF mRNA和TFPI mRNA的表达.脐动脉作为对照.结果 脐动脉外膜表达少量TF和TFPI蛋白及其mRNA,而动脉粥样硬化斑块血管增生内膜大量表达TF和TFPI蛋白及其mRNA.结论 增生内膜中所有细胞类型及细胞间质都表达TF和TFPI.

Expressions of tissue factor and tissue factor pathway inhibitor in the atherosclerotic plaque

Objective To observe the expression and location of TF and TFPI in femoral artery atherosclerotic plaque. Methods We detected the expressions and locations of TF and TFPI in femoral artery atherosclerotic plaque by immunohistochemical and double-stain immunohistochemical method. We detected TF mRNA and TFPI mRNA expressions in atherosclerotic plaque by RT-PCR, with the normal umbilical artery as a control. Results The normal umbilical artery contained little TF,TFPI and their mRNA in the adventitia. A great deal of TF, TFPI and their mRNA were found in the tunica intima of the femoral artery atherosclerotic plaque. Conclusion Expression of TF, TFPI and their mRNA of all types of cells and stroma in the proliferative tunica intima.