灵芝孢子粉对2型糖尿病大鼠附睾细胞线粒体Ca~(2+)细胞色素C的影响

目的:研究灵芝孢子粉对2型糖尿病大鼠附睾组织中细胞色素C(Cyt-C)、线粒体Ca2+的影响。方法:青春期Wistar大鼠50只,大鼠尾静脉一次性注射2%链脲佐菌素(STZ)或柠檬酸-柠檬酸钠缓冲液制备2型糖尿病大鼠模型和对照组模型。糖尿病大鼠模型随机分模型组和灵芝组,每组20只,分别给以高脂高糖饮食、高脂高糖+灵芝孢子粉250mg/(kg.d)],对照组10只,正常饮食。10周后,取双侧附睾,检测附睾细胞线粒体Ca2+、Cyt-C含量。结果:2型糖尿病模型制备成功,模型组附睾细胞线粒体Ca2+含量(3.279±0.502)mg/L]明显高于对照组(2.606±0.048)mg/L,P<0.01],灵芝组(2.693±0.196)mg/L]明显低于模型组(P<0.05),与对照组差异无显著性(P>0.05)。模型组线粒体Cyt-C含量(3.213±1.511)μmol/L]明显少于对照组(5.688±1.679)μmol/L,P<0.05],胞质Cyt-C含量(2.484±0.661)μmol/L]明显高于对照组(1.574±0.329)μmol/L,P<0.01];灵芝组线粒体Cyt-C含量(5.258±1.560)μmol/L]高于模型组,但差异无显著性(P>0.05),胞质Cyt-C含量(1.727±0.396μmol/L]明显低于模型组(P<0.05),与对照组差异无显著性(P>0.05)。结论:2型糖尿病大鼠附睾细胞钙稳态失衡、线粒体有损伤,附睾细胞可能存在细胞凋亡过度。灵芝孢子粉在糖尿病状态下,对附睾组织有保护作用或有对抗细胞凋亡作用。

Effects of Ganoderma Lucidum Spores on Mitochondrial Calcium Ion and Cytochrome C in Epididymal Cells of Type 2 Diabetes Rats

OBJECTIVE: To observe the effects of ganoderma lucidum spores (GLS) on mitochondrial calcium ion and cytochrome C in the epididymal cells of type 2 diabetes rats. METHODS: Fifty adolescent rats were randomly divided into a model group (n=20), a GLS group (n=20) and a control group (n=10). The animals of the former two groups were injected with 2% STZ via vena caudalis for one time to induce type 2 diabetes. Then the model group was given high-fat-sugar diet, the GLS group high-fat-sugar diet + GLS (250 mg/kg x d), and the control group normal diet + CA-citrate sodium buffer. The bilateral epididymides were obtained 10 weeks later and the contents of mitochondrial calcium and cytochrome C detected. RESULTS: Type 2 diabetes models were successfully constructed. The content of mitochondrial calcium in the epididymal cells was significantly higher in the model group (3.279 +/- 0.502] mg/L) than in the control group (2.606 +/- 0.048] mg/L, P < 0.01), with no significant difference between the GLS group (2.693 +/- 0. 196] mg/L) and the control (P > 0.05). In the model group, the content of mitochondrial cytochrome C (3.213 +/- 1.511] micromol/L) was significantly lower (P < 0.05) while that of cytoplasm cytochrome C (2.484 +/- 0.661] micromol/L) significantly higher (P < 0.05) than in the control (5.688 +/- 1.679] micromol/L and 1.574 +/- 0.329] micromol/L, respectively). In the GLS group, the content of mitochondrial cytochrome C (5.258 +/- 1.560] micromol/L) was higher, with no significant difference (P > 0.05), and that of cytoplasm cytochrome C (1.727 +/- 0.396] micromol/L) significantly lower than in the model group (P < 0.05), but the difference between the GLS and the control group was not significant (P > 0.05). CONCLUSION: With disequilibrium of calcium homeostasis and damage to mitochondria, there might be excessive apoptosis in the epididymal cells of type 2 diabetes rats. Ganoderma lucidum spores could protect epididymal cells and counteract their apoptosis in diabetic condition.