青海省2004年人间鼠疫分离株基因分型及流行病学意义

目的探讨鼠疫耶尔森菌（鼠疫菌）基因分型在鼠疫暴发中的流行病学意义.方法根据已经证实的23个差异区段设计引物,对2004年青海省人间鼠疫流行期间分离到的13株鼠疫菌进行PCR扩增.结果13株鼠疫菌可分为4个基因组型,即Genomovar 8、10、15和16.其中囊谦的8株菌全部为Genomovar 10;乌兰的2株菌分别为Genomovar 8和15;祁连、曲麻莱、称多的3株鼠疫菌的基因组型均为Genomovar 16.结论鼠疫菌基因分型是鼠疫疫情暴发流行病学调查的有力工具.

A molecnlar epidemiological study on human plague fulminant epidemic in Qinghai, 2004

Objective To study the epidemiology of genotyping Yersinia pestis isolated in the fulminant epidemics of human plague in Qinghai province in 2004. Methods Primer pairs targeting the twenty-three different identified regions(DFRs) were designed to detect the presence or deletion of each DFR in 13 strains of Yersinia pestis isolated from the fulminant epidemic of human plague in Qinghai province in 2004. Results There were 4 genomovars,i.e. Genomovar 8,10,15 and 16 in the 13 strains of Yersinia pestis identified. The genomovar of all the strains of Yersinia pestis isolated from Nangqian county was Genomovar 10. Among the two strains of Yersinia pestis isolated from Wulan county, the genomovar of one strain was Genomovar 8 and the other was Genomovar 10. The genomovars of all the strains of Yersinia pestis isolated from Qilian, Qumalai and Chengduo county belonged to Genomovar 16. Conclusion It was demonstrated that the genotyping of Yersinia pestis appeared to be a powerful tool for investigating human plague epidemics.