| 宫颈癌组织细胞分化抑制因子-1表达及其意义 |
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| 引用本文： | 渠力平,于小玲. 宫颈癌组织细胞分化抑制因子-1表达及其意义[J]. 齐鲁医学杂志, 2011, 26(6) |
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| 作者姓名： | 渠力平 于小玲 |
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| 作者单位： | 1. 青岛大学医学院附属烟台毓璜顶医院妇产科,山东烟台264000;青岛大学医学院病理生理学教研室,山东烟台264000
2. 青岛大学医学院病理生理学教研室,山东烟台,264000 |
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| 摘 要： | 目的 了解细胞分化抑制因子-1 (Id-1) mRNA和蛋白在人宫颈癌组织中的表达及其意义.方法 分别应用荧光实时定量逆转录-聚合酶链反应和免疫组织化学方法,检测Id-1 mRNA和蛋白在6例人正常宫颈、6例宫颈上皮内瘤样变(CIN)和24例宫颈癌组织中的相对表达定量,分析Id-1表达与宫颈癌病理参数间的相关性.结果 Id-1蛋白在正常宫颈组织未见阳性表达,在CIN组织中有少量表达,在宫颈浸润癌组织中呈明显的阳性表达,宫颈浸润癌组织Id-1蛋白阳性面积百分比和阳性染色吸光度均显著高于CIN组织(t=7.80、3.15,P＜0.05).正常宫颈组织、CIN组织和宫颈癌组织Id-1 mRNA相对定量依次增高,差异有显著性(F=3 030.30,q=17.78、95.04,P＜0.01).宫颈癌组织中Id-1 mRNA和蛋白表达呈正相关(r=0.89,P＜0.01).Id-1表达和宫颈癌组织分级及FIGO分期呈正相关(r=0.67、0.58,P＜0.01、0.05).结论 检测Id-1表达有助于宫颈癌的辅助诊断.
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| 关 键 词： | 宫颈肿瘤 分化抑制因子-1 逆转录聚合酶链反应 免疫组织化学 |
EXPRESSION OF CELL DIFFERENTIATION INHIBITING FACTOR-1 IN TISSUE OF CERVICAL CANCER |
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| QU Liping,YU Xiaoling. EXPRESSION OF CELL DIFFERENTIATION INHIBITING FACTOR-1 IN TISSUE OF CERVICAL CANCER[J]. Medical Journal of Qilu, 2011, 26(6) |
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| Authors: | QU Liping YU Xiaoling |
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| Affiliation: | QU Liping,YUXiaoling(Department of Obstetrics and Gynecology,The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College,Yantai 264000,China) |
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| Abstract: | Objective To study the expressions of cell differentiation inhibiting factor-1(Id-1) mRNA and protein in human cervical cancer and its significance.Methods The quantity of Id-1 mRNA and protein were detected by real time RT-PCR and immunohistochemical method,respectively,in six samples of normal cervix,six of cervical intraepithelial neoplasia(CIN) and 24 of cervical cancer.The relationship between Id-1 and some clinical parameters was analyzed.Results No positive expressions of Id-1 mRNA and protein were s... |
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