Novel subdivisions of the rat accessory olfactory bulb revealed by the combined method with lectin histochemistry,electrophysiological and optical recordings |
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| Institution: | 1. Department for Oral and Maxillofacial Plastic Surgery and Interdisciplinary Department of Oral Surgery and Implantology, University of Cologne, Germany;2. Department of Prosthodontics, University of Erlangen, Germany;3. Department for Oral and Maxillofacial Surgery, Red Cross Hospital Kassel, Germany;1. Department of Psychology, Georgetown University, Washington, DC, USA;2. Interdisciplinary Program in Neuroscience, Georgetown University, Washington, DC, USA;3. Department of Psychology, University of Pennsylvania, Philadelphia, PA, USA;4. National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA;5. Department of Psychology, Louisiana State University, Baton Rouge, LA, USA;6. National Institute of Nursing Research, National Institutes of Health, Bethesda, MD, USA;7. Department of Neurology, Georgetown University Medical Center, Washington, DC, USA;1. Laboratory of Animal Behavior, Physiology and Welfare, Animal and Food Sciences Department of Animal Science, Tech University. Box 42141 Lubbock, TX 79409-2141, United States;2. Department of Animal and Food Sciences, Box 42141 Texas Tech University, Lubbock, TX 79409-214, United States |
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| Abstract: | Wistaria floribunda agglutinin and peanut agglutinin were found to bind histochemically to the anterior and posterior regions, respectively, of the vomeronasal nerve and glomerular layers in the rat accessory olfactory bulb. Furthermore, Ricinus communis agglutinin showed strong binding to the anterior region of the vomeronasal nerve and glomerular layers, whereas it bound weakly and/or moderately to the rostral two-thirds of the posterior glomerular layer but not at all to the caudal one-third. This suggests that the posterior region is further divided into two subregions. An electrophysiological mapping study in sagittal slice preparations demonstrated that stimulation given within the anterior vomeronasal nerve layer elicited field potentials within the anterior region of the external plexiform layer, whereas shocks to the rostral two-thirds and the caudal one-third of the posterior vomeronasal nerve layer provoked field responses within the rostral two-thirds and within the caudal one-third of the posterior external plexiform layer, respectively, indicating that the posterior external plexiform layer is also divided into two subregions. Real-time optical imaging showed similar results as above, except that neural activity also spread into mitral cell layers. Furthermore, the most anterior and posterior ends of the neural activity evoked in the rostral two-thirds of the posterior region immediately adjoined the posterior border of that evoked in the anterior region and the anterior border of that evoked in the caudal one-third of the posterior region, respectively. Moreover, the granule cell layer was also found to have similar boundaries. Thus, optical imaging studies demonstrated individual precise boundaries of these subdivisions, which were positioned right beneath those defined by Ricinus communis agglutinin histochemistry.The presence of functional segregation in each layer leads us to conclude that there are at least three different input–output pathways in the rat vomeronasal system. |
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