γ-干扰素对前列腺癌细胞粘附和侵袭能力影响的初步研究

目的：探讨γ-干扰素对前列腺癌细胞粘附和侵袭行为的影响。方法：用纤维粘连蛋白和层粘连蛋白处理细胞培养板，检测γ-干扰素对前列腺癌细胞系LNCaP和PC-3粘附作用的影响；用Transwell小室，以Matrigel和纤维粘连蛋白构建基底膜，检测γ-干扰素对前列腺癌细胞侵袭人工基底膜能力的影响；应用Western-blot法检测γ-干扰素对前列腺癌细胞膜粘连蛋白-2（annexin-2）表达的影响。结果：γ-干扰素未处理的两种人前列腺癌细胞系细胞LNCaP、PC-3粘附率分别为46％和40％，γ-干扰素处理的两种细胞系细胞LNCaP、PC-3粘附率分别为21％和23％，同种细胞系γ-干扰素处理组与未处理组间差异有显著性（P均〈0．05）。在相同细胞系γ-干扰素处理组较未处理组前列腺癌细胞24h侵袭能力明显降低（P均〈0．05）。Western印迹结果表明γ-干扰素可显著抑制annexin-2蛋白的表达（P〈0．05）。结论：γ-干扰素可能通过下调annexin-2的表达来抑制前列腺癌细胞的粘附和侵袭。

Interferon-gamma decreases the adhesiveness and invasiveness of prostate cancer cells

OBJECTIVE: To study the effects of interferon-gamma (IFN-gamma) on the adhesiveness and invasiveness of prostate cancer cells. METHODS: We determined the effect of IFN-gamma on the adhesiveness of prostate cancer cell lines LNCaP and PC-3 by treating the plate with fibronectin and laminin, on the invasiveness of prostate cancer cells into the artificial basal membrane consisting of Matrigel and fibronectin using the Transwell chamber, and on the expression of annexin-2 in these cells by Western blot. RESULTS: The adhesion rates of prostate cancer cell lines LNCaP and PC-3 were 21% and 23% in the IFN-gamma treatment group, significantly lower than 46% and 40% in the untreated group (P < 0.05). The invasiveness of the cells was markedly decreased in the former as compared with the latter (P < 0.05). IFN-gamma significantly inhibited the expression of annexin-2 in prostate cancer cell lines LNCaP and PC-3 (P < 0.05). CONCLUSION: IFN-gamma can decrease the adhesiveness and invasiveness of the prostate cancer cell line by down-regulating the expression of annexin-2.