镉对人胚肾细胞中经典瞬时受体电位通道mRNA表达影响

目的观察不同质量浓度的氯化镉(CdCl2)染毒后人胚肾细胞(HEK 293T细胞)经典瞬时受体电位通道(TRPC)6个亚型mRNA表达水平的改变,在mRNA水平上探讨镉对肾细胞钙通道的影响。方法 HEK 293T细胞分别暴露于7.5、15.0、30.0、45.0和60.0μmol/L的CdCl28 h后,应用四甲基偶氮唑蓝(MTT)比色法测定细胞增殖活力;实时荧光定量聚合酶链反应法检测0、15.0和30.0μmol/L CdCl2作用8 h和30.0μmol/L CdCl2染毒4 h后TRPC 6个亚型mRNA表达水平。结果以对照组(CdCl20μmol/L)HEK 293T细胞存活率为100%,各不同质量浓度的染毒组细胞存活率分别为79.40%、77.29%、72.26%、70.25%、60.80%,与对照组比较,差异有统计学意义(P<0.05或P<0.01)。经CdCl2染毒后,各染毒组HEK 293T细胞的TRPC 6个亚型mRNA相对表达量均低于对照组(P<0.05或P<0.01)。结论 CdCl2可能引起HEK 293T细胞的TRPC基因mRNA的表达水平发生改变。

Effects of cadmium on expressions of TRPC mRNA in human embryo kidney cells

Objective To study the effects of cadmium chloride(CdCl2) on the expressions of transient receptor potential canonical(TRPC) mRNA in human embryo kidney cells(HEK 293T),and to discuss the effects of CdCl2 on the calcium channel in HEK 293T cells.Methods HEK 293T cells were exposed to CdCl2 for 8 hours at the mass concentrations of 7.5,15.0,30.0,45.0 and 60.0 μmol/L respectively.The cell viability was measured with MTT colorimetric assay.The expressions of TRPC1,TRPC3,TRPC4,TRPC5,TRPC6 and TRPC7 mRNA were determined in HEK 239T cells after exposure to 0,15.0,30.0 μmol/L CdCl2 for 8 hours and 30.0 μmol/L CdCl2 for 4 hours by quantitative RT-PCR.Results Compared with the control group(CdCl2 0 μmol/L),the survival rate of HEK 293T cells were 79.40%,77.29%,72.26%,70.25%,60.80%(P<0.05 or P<0.01) respectively at the mass concentrations of 7.5,15.0,30.0,45.0 and 60.0 μmol/L CdCl2 after 8 h exposure.The mRNA expressions of TRPC(TRPC1,TRPC3,TRPC4,TRPC5,TRPC6,TRPC7) were decreased after treated with CdCl2(P<0.05 or P<0.01).Conclusion CdCl2 may be able to change the expressions of TRPC mRNA in HEK 293T cells.