HPLC同时测定参附汤中人参皂苷Rg_1、Re、Rb_1含量的研究

目的：建立参附汤中人参皂苷Rg1、Re、Rb1的含量测定方法。方法：采用Inertsil ODS–SP（4.6mm×250mm,5μm）色谱柱,柱温25℃,流动相为乙腈：水,梯度洗脱,流速：1.0mL.min-1,检测波长203nm。结果：人参皂苷Rg1在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率98.86%,RSD为2.32%;人参皂苷Re在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率99.09%,RSD为2.22%;人参皂苷Rb1在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率99.53%,RSD为1.68%;结论：该方法学考察符合定量要求,结果准确,可用于同时测定参附汤中人参皂苷Rg1,Re,Rb1的含量,为参附汤的质量控制提供依据。

Simultaneous Determination of Ginsenoside Rg_l,Re,Rb_1 in Shenfu Decoction by HPLC

Objective： To establish a method for the determination of ginsenoside Rg1,Re,Rb1 in Shenfu Decoction.Method： With a Inertsil ODS SP column（4.6mm 250mm,5μm） at 25℃,the mobile phase consisted of acetonitrile and water for gradient elution with the flow rate at 1.0 mL·min-1.The detection wavelength was 203nm.Result： The resolutions of the determined peaks were fine.The linearity ranges for ginsenoside Rgl,Re,Rbl were 12.5~500μg·mL-1（r=0.9999）,12.5~500μg·mL-1（r=0.9999）and 12.5~500μg·mL-1g（r=0.9999）,respectively.The average recoveries were 98.86% with RSD 2.32%,99.09% with RSD 2.22%,99.53% with RSD 1.68%.Conclusion： The quantitative method for the determination was feasible and beneficial for the quality control of Shenfu Decoction.