| 黄芩苷对肝癌细胞SMMC-7721 JAK-STAT信号通路STAT3的影响 |
| |
| 引用本文： | 郭昱,霍瑞静,姚金锋.黄芩苷对肝癌细胞SMMC-7721 JAK-STAT信号通路STAT3的影响[J].世界华人消化杂志,2011(22). |
| |
| 作者姓名： | 郭昱 霍瑞静 姚金锋 |
| |
| 作者单位： | 河北医科大学第二医院; |
| |
| 基金项目： | 河北省科技支撑计划基金资助项目,No.072461478D~~ |
| |
| 摘 要： | 目的:探讨黄芩苷对肝癌细胞SMMC-7721JAK-STAT信号通路STAT3的影响.方法:将肝癌细胞SMMC-7721分为4组:对照组、黄芩苷组、AG490组、黄芩苷+AG490组.应用RT-PCR法检测各组肝癌细胞SMMC-7721中STAT3mRNA表达,Westernblot法检测肝癌细胞SMMC-7721中STAT3、P-STAT3蛋白表达.结果:黄芩苷可以下调肝癌细胞SMMC-7721STAT3mRNA表达,与对照组比较明显下降(0.505±0.111vs0.697±0.145,P<0.05);并可以降低STAT3蛋白的表达量(0.879±0.012vs1.087±0.015,P<0.05);还可以抑制STAT3向活化形式P-STAT3转化,与对照组比较P-STAT3表达明显下降(0.983±0.085vs1.103±0.074,P<0.05),而与AG490联合应用后P-STAT3蛋白表达量较单用黄芩苷下降明显(0.756±0.103vs0.983±0.085,P<0.05).结论:黄芩苷能下调STAT3mRNA表达水平,降低STAT3蛋白表达,还可以抑制STAT3向活化形式P-STAT3转化,...
|
| 关 键 词： | 黄芩苷 肝癌 JAK-STAT信号通路 STAT3 AG490 |
Effect of baicalin on the JAKSTAT3 signaling pathway in human hepatocellular carcinoma cell line SMMC-7721 |
| |
| Yu Guo,Rui-Jing Huo,Jin-Feng Yao,the Second Hospital of Hebei Medical University,Shijiazhuang ,Hebei Province,China.Effect of baicalin on the JAKSTAT3 signaling pathway in human hepatocellular carcinoma cell line SMMC-7721[J].World Chinese Journal of Digestology,2011(22). |
| |
| Authors: | Yu Guo Rui-Jing Huo Jin-Feng Yao the Second Hospital of Hebei Medical University Shijiazhuang Hebei Province China |
| |
| Institution: | Yu Guo,Rui-Jing Huo,Jin-Feng Yao,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China |
| |
| Abstract: | AIM:To investigate the effect of baicalin on the JAK-STAT3 signaling pathway in human hepatocellular carcinoma cell line SMMC-7721.METHODS:SMMC-7721 cells were divided into four groups:blank control group,baicalin group,AG490 group,and baicalin plus AG490 group.The mRNA expressional level of STAT3 in SMMC-7721 cells was measured by RT-PCR.The protein expression levels of STAT3 and p-STAT3 in SMMC-7721 cells were measured by Western blot.RESULTS:The expressional level of STAT3 mRNA in SMMC-7721 cells was sig... |
| |
| Keywords: | Baicalin Hepatocellular carcinoma JAK-STAT pathway STAT3 AG490 |
| 本文献已被 CNKI 等数据库收录！ |
|
