| PAP、双桥PAP和ABC法敏感性及操作方法的比较 |
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| 引用本文： | 刘开凤,叶诸榕. PAP、双桥PAP和ABC法敏感性及操作方法的比较[J]. 四川大学学报(医学版), 1987, 0(1) |
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| 作者姓名： | 刘开凤 叶诸榕 |
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| 作者单位： | 华西医大病理学教研室(刘开凤)，上海医科大学病理解剖学教研室(叶诸榕) |
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| 摘 要： | 作者应用四种抗血清,在不同孵育条件下比较PAP、双桥PAP及ABC法的敏感性。双桥PAP法为一实用、敏感的免疫组化法。探讨了提高免疫组化染色强度的操作步骤:用延长第一抗体孵育时间(过夜),抗体稀释度提高5～10倍,第二、三步骤用提高孵育温度(37℃)来缩短孵育时间,可获得满意的染色效果。
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| 关 键 词： | PAP法 双桥PAP法 ABC法 S-100蛋白 生长激素 胶质纤维酸性蛋白 |
A COMPARATIVE STUDY OF SENSITIVITY OF PAP,DOUBLE--BRIDGE PAP AND ABC METHOD WITH FOUR ANTIBODIES IN MOIST CHAMBER AND ON HOTPLATE |
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| Liu Kaifeng,Ye Zhurong. A COMPARATIVE STUDY OF SENSITIVITY OF PAP,DOUBLE--BRIDGE PAP AND ABC METHOD WITH FOUR ANTIBODIES IN MOIST CHAMBER AND ON HOTPLATE[J]. Journal of Sichuan University. Medical science edition, 1987, 0(1) |
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| Authors: | Liu Kaifeng Ye Zhurong |
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| Affiliation: | Liu Kaifeng,Ye Zhurong Department of Pathology Department of Pathology,Shanghai Medical University |
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| Abstract: | |
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| Keywords: | PAP method Double-bridge PAP method ABC method S-100 protein Growth hormone Glial fibrillary acidic protein |
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