南京地区汉族人群TRAIL基因多态性与前列腺癌的易感性研究

目的:探讨南京地区汉族人群中肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因多态性与前列腺癌(PCa)易感性的关系。方法:采用病例对照研究,提取187例PCa患者和237例非PCa健康人(对照组)外周血基因组DNA,应用聚合酶链反应-连接酶特异检测技术(PCR-LDR)分析186例PCa患者和237例对照组TRAIL基因-716位点的多态性,比较不同基因型与PCa易感性的关系。结果:TRAIL基因启动子区存在一个SNP位点(-716A/G),基因型分别为AA型、AG型和GG型;Logistic回归分析显示,携带AG、GG和AG+GG基因型的个体与PCa发病风险之间无明显相关性(OR=0.89,95%CI=0.54～1.47;OR=0.94,95%CI=0.69～1.27;OR=0.87,95%CI=0.54～1.41)。结论:中国南京地区汉族人群中TRAIL基因-716位点基因多态性对PCa易感性无明显影响。

TRAIL gene polymorphism and genetic susceptibility to prostate cancer in the Chinese Han population of Naniin2

Objective：To investigate the correlation between the polymorphism of the tumor necrosis factor-related apoptosis inducing ligand（TRAIL） and the genetic susceptibility to prostate cancer（PCa） in the Chinese Han population in Nanjing.Methods：We performed a case control study on 187 cases of PCa and 237 cancer-free healthy controls.Peripheral blood genome DNA was extracted from the subjects for analysis of the polymorphism of the TRAIL-716 locus by polymerase chain reaction-ligase detection reaction（PCR-LDR）.The correlations between the susceptibility to PCa and different genotypes were compared.Results：An SNP（-716A/G） was found in the promoter of the TRAIL gene.AA,AG and GG genotypes were identified.Logistic regression analysis suggested that AG,GG and AG＋GG genotypes had no significant correlation with the risk of PCa（OR=0.89,95%CI=0.54-1.47;OR=0.94,95%CI=0.69-1.27;OR=0.87,95%CI=0.54-1.41）.Conclusion：The TRAIL-716 polymorphism is not directly related with the genetic susceptibility to PCa in the Chinese Han population of Nanjing.