中药芪芍五味合剂对病毒性心肌炎小鼠心肌细胞凋亡和坏死的影响

取 2 4 0只 4周龄 Balb/ c雄性小鼠 ,随机等分为 6组 (每组 4 0只 ) ,即 :正常对照组 (A组 )、中药对照组 (B组 )、病毒对照组 (C组 )、中药治疗组 (D组 )、维生素 C治疗组 (E组 )、病毒唑治疗组 (F组 )。 A、B两组每只小鼠均腹腔接种 0 .1ml不含病毒的 Eagle's液 ,C～F组每只小鼠服腔接种 0 .1ml10 7.5TCID/ ml的柯萨奇 B3(CVB3)病毒液。根据分组分别给予不同的药物治疗。结果 :病毒性心肌炎时心肌细胞的凋亡率和坏死率均显著增加。心肌细胞凋亡高峰在CVB感染后 7～ 10天 ,而心肌坏死高峰出现在 CVB3感染后 14天 ,并且与组织形态学观察一致。应用中药黄芪、赤芍治疗后 ,心肌细胞凋亡率和坏死率均显著降低 (P <0 .0 5 ) ,维生素 C和病毒唑均可降低心肌细胞坏死率 ,但对心肌细胞凋亡影响不大。 CVB感染 3～ 7天内 ,各感染组心肌细胞凋亡率和坏死率均与心肌内病毒滴度呈正相关 (r =0 .76、P <0 .0 1,r=0 .5 4、 P <0 .0 5 ) ;而感染 10～ 30天 ,凋亡率及坏死率与病毒含量无相关性。各感染组心肌细胞凋亡率与坏死率均与心肌病理积分呈正相关 (r=0 .5 5、P <0 .0 5 ,r=0 .76、P<0 .0 1)。中药黄芪、赤芍、五味子可明显减轻病毒性心肌炎小鼠的病理损害程度 ,显著降低病毒性心肌炎心肌细胞凋亡率和坏死率 ,对

Effects of Chinese herbs on cardiomyocytes cell apoptosis and necrosis of mice with viral myocarditis

To study the effect of Chinese herbs Astragalus membranaceous,Redpeony root and Chinese magnoliavine fruit on myocardial cell apoptosis and necrosis with viral myocarditis(VM),240 male Balb/c mice,4 weeks old,were divided into 6 groups randomly Group A and Group B mice were inoculated intraper itoneally with Eagle's medium 0 1 ml Group C,D,E,F were inoculated intrapeirtoneally of 10 7 5 TCID50 /ml CVB 3 at a dose of 0 1 ml per mouse The percentage of apoptosis and necrosis of cardiac myocytes was examined with Annexin V/PI technique In Chinese herbs group(group D),the percentage of apoptosis and necrosis in cardiomyocytes was significantly lower than that in viral group(group C) In VitC and rabavirin groups(group E,F),the percentage of myocytes necrosis was significantly lower than that in groupc,but the percentage of myocytes apoptosis wasn't significantly lower On day 3~7,the percentage of myocytes apoptosis and necrosis was positively correlated with myocardial histopathological score Chinese herbs therapy ameliorated the severity of myocarditis,its effect was more significantly than that of VitC and rabavirin