| 脐带间充质干细胞移植治疗大鼠重症肌无力的研究 |
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| 引用本文： | Yu JX,Chen F,Sun J,Wang JM,Zhao QJ,Ren XJ,Ma FX,Yang SG,Han ZB,Han ZC. 脐带间充质干细胞移植治疗大鼠重症肌无力的研究[J]. 中国实验血液学杂志, 2011, 19(3): 744-748 |
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| 作者姓名： | Yu JX Chen F Sun J Wang JM Zhao QJ Ren XJ Ma FX Yang SG Han ZB Han ZC |
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| 作者单位： | 中国医学科学院、北京协和医学院血液学研究所、血液病医院,实验血液学国家重点实验室,天津,300020 |
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| 基金项目： | 国家重点基础研究发展计划(编号2011CB964800); 国家自然科学基金(编号30900557) |
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| 摘 要： | 脐带间充质干细胞因其来源广泛,易扩增,低免疫原性等特点被人们越来越多地应用于疾病治疗的研究中。本研究旨在为重症肌无力疾病探求一种新的应用干细胞的治疗方法。将脐带间充质干细胞(UCMSC)移植到重症肌无力大鼠中,应用免疫荧光法观察人源化细胞的分布,ELISPOT法观察MSC对B细胞原位分泌抗体的影响,Transwell试验检测分泌IFN-γ的水平。结果发现,经静脉输注的UCMSC能快速的迁移到炎症部位和局部淋巴结,而且在淋巴结的髓质区也可以检测到人源化的细胞。体外原位检测乙酰胆碱受体(AchR)抗体分泌的试验发现,当MSC与淋巴结来源的淋巴细胞充分接触,能有效地抑制AchR抗体的产生。Transwell试验显示,UCMSC与CD4T细胞直接接触,能有效地降低IFN-γ的产生,在一定程度上缓解重症肌无力体内的Th1/Th2细胞失衡的免疫状态。结论 :在重症肌无力大鼠中,MSC可能通过相互接触或/和释放细胞因子而调节机体的免疫反应,这为重症肌无力提供了一种潜在的治疗新途径。
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| 关 键 词： | 间充质干细胞 重症肌无力 免疫调节 |
Umbilical cord mesenchymal stem cell transplantation for treatment of experimental autoimmune myasthenia gravis in rats |
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| Yu Jing-Xia,Chen Fang,Sun Jun,Wang Ji-Ming,Zhao Qin-Jun,Ren Xin-Jun,Ma Feng-Xia,Yang Shao-Guang,Han Zhi-Bo,Han Zhong-Chao. Umbilical cord mesenchymal stem cell transplantation for treatment of experimental autoimmune myasthenia gravis in rats[J]. Journal of experimental hematology, 2011, 19(3): 744-748 |
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| Authors: | Yu Jing-Xia Chen Fang Sun Jun Wang Ji-Ming Zhao Qin-Jun Ren Xin-Jun Ma Feng-Xia Yang Shao-Guang Han Zhi-Bo Han Zhong-Chao |
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| Affiliation: | YU Jing-Xia,CHEN Fang,SUN Jun,WANG Ji-Ming,ZHAO Qin-Jun,REN Xin-Jun,MA Feng-Xia,YANG Shao-Guang,HAN Zhi-Bo,HAN Zhong-Chao State key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,Chinese Academy of Medical Sciences & Peking Union Medical College,Tianjin 300020,China |
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| Abstract: | Umbilical cord mesenchymal stem cell(UCMSC) transplantation has been widely used in the treatment of a variety of diseases due to their advantages such as abundant resources,low immunogenicity and large ex vivo expansion capacity.This study was aimed to investigate the effects of UCMSC on experimental autoimmune myasthenia gravis(EAMG) rats.The distribution of human-derived cells was observed by immunofluorescence method,the effect of MSC on B-cell in situ-secreted antibodies was assayed by ELISPOT,the secr... |
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| Keywords: | mesenchymal stem cells experimental autoimmune myasthenia gravis immunomodulation |
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