冻干血小板对大鼠难愈合创伤模型的实验治疗

血小板含有20多种生长因子,对于创伤尤其是难愈性创伤具有治疗作用。本研究以糖尿病SD大鼠为基础,建立了一种难愈合创口模型,用于评价冻干血小板对难愈性创伤修复的治疗作用。采用链脲佐菌素(Streptozotocin,STZ)65mg/kg对大鼠腹腔注射制备糖尿病模型。取正常大鼠10只,糖尿病大鼠20只,将其分为正常对照组(NDR)、糖尿病对照组(DR)和冰冻干燥血小板治疗组(TLP);在各组大鼠背部造成直径2cm圆形创面,分别于第l、3、5、7、12天进行创面照相和透明膜描计法计算创面愈合速度。结果显示,注射STZ72小时后糖尿病组血糖明显高于16.7mmol/L。1周后糖尿病大鼠体重与正常对照组比较明显减轻(p<0.05)。糖尿病对照组创口的愈合速度在各个时间点均低于正常对照组创口(p<0.05),冰冻干燥血小板治疗组创口愈合速度显著快于糖尿病对照组。第12天时,正常对照组和治疗组创口再上皮化率分别达到88.1%和81.8%,而糖尿病组只有62.8%。结论 :以链脲佐菌素建立的糖尿病大鼠能成功制备难愈合创口模型,冰冻干燥血小板对该难愈性创伤模型具有治疗作用。

Healing effect of lyophilized platelets on rat chronic wound model

Platelets carry over 20 growth factors,which all have been shown to improve wound healing,particularly recalcitrant wound healing.The aim of this study was to investigate the healing effect of lyophilized platelets on the chronic wounds through establishing diabetic rat chronic wound model.Healthy male SD rats were intraperitoneally injected with streptozotocin(STZ) solution at the dose of 65 mg/kg.The blood glucose and weights were observed every week.The re-epithelialization rates of normal control group(...