GSTP1、CYP2E1基因遗传多态性与急性白血病易感性的关系

本研究旨在探讨谷胱甘肽巯基转移酶P1(GSTP1)和细胞色素P4502E1(CYP2E1)基因多态性与急性白血病易感性的关系。采用1∶1配对病例-对照、LDR分型方法对150例急性白血病(AL)患者和150例对照组进行GSTP1和CYP2E1的基因多态性进行检测。结果表明:AL病例组GSTP1基因G等位基因频率(26.7%)和Ile/Val和Val/Val基因型频率(44%)均高于对照组(10%和16%)。携带突变基因型(Ile/Val和Val/Val)个体发生AL的相对风险度为Ile/Ile个体的3.226倍(95%CI=1.527-5.236)。进一步分层分析表明,急性髓系白血病(AML)病例组Ile/Val和Val/Val基因型频率(55.0%)高于对照组(16%)(p<0.05)。携带Ile/Val和Val/Val基因型的个体发生AML的相对风险度为野生基因型(Ile/Ile)个体的2.214倍(95%CI=1.009-3.260)。AL病例组CYP2E1基因C2等位基因频率(16.7%)和C1C2/C2C2基因型频率(30%)均高于对照组(13.9%和26%),但其差异均无统计学意义。进一步分层分析...

Relation of GSTP1 and CYP2E1 polymorphisms with susceptibility to acute leukemia

This study was aimed to investigate the relation of glutathione S-transferase pI （ GSTP1 ） and cytochrome P450 enzyme 2El （CYP2E1） gene polymorphisms with the susceptibility to acute leukemia （AL） in Chinese population. The GSFP1 and CTP2E1 gene polymorphisms in 150 patients with AL and 150 patients with non-hematological diseases or non-tumor as controls were detected by means of case-control paired 1 ： 1 method and ligase detection reaction （LDR） techniques. The results indicated that the frequently of G allele and lle/Val ＋ Val/Val of GSTP1 gene （26.7% and 44% respectively） in AL group were higher than those in control group （10% and 16% respectively） ; the AL risk for persons with Ile/Val ＋ Val/Val was 3. 260-fold （95% CI = 1. 527 -5. 236） of persons with lie/lie. The further stratified analysis showed the frequency of lle/Val ＋ Val/Val in AML group was higher than that in control group （55% vs 16%, p 〈0.05） ; the AML risk for persons with lle/Val ＋ Val/Val was 2. 214-fold （95% CI = 1. 009 - 3. 260） as persons with lie/lie. The frequencies of C2 allele（ 16.7% ） and CIC2/C2C2 of CYP2E1 gene （30%） in AL group seemed higher than those in control groups （ 13.9% and 26% ）, but the difference between them was not statistical significant （p 〉0.05）. The further stratified analysis showed that CIC2/C2C2 of CYP2E1 gene occurred more frequently in AML group （36%） than that in control group （32%） , but there was no statistical difference between them （p 〉 0.05 ）. Combined genotype analysis showed that the AML risk for persons in combination of Ue/Val ＋ Val/Val of GSTP1 gene with C1C2 ＋ C2C2 of CYP2E1 gene increased by 3. 208-fold. It is concluded that the GSTP1 gene is related with susceptibility to AML, the AL risk for persons with lle/Val ＋ Val/Val of GSTP1 gene decreased, while CYP2E1 gene is not related with susceptibility to AL, the AML risk for persons in combination of GSTP1 wildtype with CYP2E1 hybrid and mutant genotype can be further decreased.