| 自噬相关基因Beclin1和MAPLC3在急性白血病骨髓单个核细胞中的表达及其意义 |
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| 引用本文： | Hu XY,Bai H,Pan YZ,Wnag CB,Wu B,Zhao Q,Ai H,Chen Z,Han X. 自噬相关基因Beclin1和MAPLC3在急性白血病骨髓单个核细胞中的表达及其意义[J]. 中国实验血液学杂志, 2011, 19(3): 598-601 |
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| 作者姓名： | Hu XY Bai H Pan YZ Wnag CB Wu B Zhao Q Ai H Chen Z Han X |
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| 作者单位： | 1. 兰州军区兰州总医院血液科,甘肃兰州,730050;兰州大学第二临床医学院,甘肃兰州,730000
2. 兰州军区兰州总医院血液科,甘肃兰州,730050 |
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| 摘 要： | 本研究旨在检测人急性白血病(AL)骨髓单个核细胞中自噬相关基因Beclin1和微管相关蛋白质轻链3(MAPLC3)的表达,并探讨其意义。采用透射电子显微镜(TEM)和RT-PCR方法检测初治、复发难治、完全缓解(CR)的急性白血病患者和正常人骨髓单个核细胞(BMMNC)中的自噬活性以及Beclin1、MAPLC3mRNA的表达。结果表明:急性白血病初治组骨髓单个核细胞自噬活性、Beclin1和MAPLC3mRNA表达分别为80%、0.68±0.18、0.24±0.06;在难治复发组分别为100%、0.79±0.09、0.30±0.07;在完全缓解组分别为40%、0.52±0.15、0.16±0.04;在正常对照组分别为20%、0.57±0.13、0.16±0.05。初治组和难治复发组自噬活性和Beclin1、MAPLC3mRNA的相对表达量均高于正常对照组,差异有统计学意义(p<0.05)。完全缓解组和正常对照组之间比较差异无统计学意义(p>0.05)。结论 :初治组急性白血病骨髓单个核细胞中自噬活性和自噬相关基因Beclin1、MAPLC3mRNA的表达均上调,在难治复发组自噬活性和自噬相关基因Beclin1、MAPLC3mRNA的表达上调尤为明显,这可能与急性白血病的发生、发展及其耐药相关。
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| 关 键 词： | 急性白血病 自噬相关基因 Beclin1基因 MAPLC3基因 骨髓单个核细胞 |
Expression of autophagy related gene Beclin1 and MAPLC3 in bone marrow mononuclear cells isolated from acute leukemia patients and its significance |
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| Hu Xiao-Yan,Bai Hai,Pan Yao-Zhu,Wnag Cun-Bang,Wu Bing,Zhao Qiang,Ai Hao,Chen Zhe,Han Xia. Expression of autophagy related gene Beclin1 and MAPLC3 in bone marrow mononuclear cells isolated from acute leukemia patients and its significance[J]. Journal of experimental hematology, 2011, 19(3): 598-601 |
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| Authors: | Hu Xiao-Yan Bai Hai Pan Yao-Zhu Wnag Cun-Bang Wu Bing Zhao Qiang Ai Hao Chen Zhe Han Xia |
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| Affiliation: | HU Xiao-Yan1,2,BAI Hai1,PAN Yao-Zhu1,WNAG Cun-Bang1,WU Bing1,ZHAO Qiang1,AI Hao1,CHEN Zhe1,HAN Xia11Department of Hematology,Lanzhou General Hospital,Lanzhou Military Area,Lanzhou 730050,Gansu Province,China,2Lanzhou University Second Clinical Medical College,Lanzhou 730000 |
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| Abstract: | This study was purposed to detect the expression of autophagy-related gene Beclin1 and microtubule-associated protein 1 light chain 3(MAPLC3) in bone marrow mononuclear cells(BMMNC) isolated from acute leukemia(AL) patients,and to explore its significance.Transmission electron microscopy and RT-PCR were used to detect the autophagy activity and the expression level of Beclin1 and MAPLC3 mRNA in BMMNC isolated from 27 AL patients with de novo,refractory or relapse AL and completely remission and 31 normal pe... |
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| Keywords: | acute leukemia autophagy Beclin1 MAPLC3 |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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