| 马钱子碱对人白血病细胞株K562细胞的诱导凋亡作用 |
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| 作者姓名： | Wang HL We W Ji AF Shen XL Zhang GX Zhang MX Zhai CY |
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| 作者单位： | 1. 山西医科大学临床一系,山西太原,030001
2. 长治医学院附属和平医院血液内科,山西长治,046000 |
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| 基金项目： | 国家自然科学基金资助项目(编号30950015); 山西省留学回国人员重点科研资助项目(编号2009-5) |
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| 摘 要： | 本研究旨在观察马钱子碱对人白血病细胞株K562细胞的诱导凋亡作用。应用四甲基偶氮唑蓝(MTT)法检测马钱子碱对K562细胞生长抑制作用;应用AO/EB荧光染色、Annexin-V/PI细胞凋亡检测法、DNA-ladder片段凝胶电泳法检测马钱子碱对K562细胞凋亡的影响。结果显示:马钱子碱能有效抑制K562细胞的生长,在一定范围内,抑制率随马钱子碱浓度的增高而增高,马钱子碱浓度为400μg/ml作用72小时后对K562细胞抑制作用最为明显,抑制率达94.0%;K562细胞经浓度为400μg/ml马钱子碱作用72小时后大部分细胞核染色质被EB染成桔红色并呈固缩状或圆珠状显示为晚期凋亡的细胞形态;马钱子碱作用72小时可诱导K562细胞凋亡,在马钱子碱浓度为50-400μg/ml范围内随药物浓度的增加,细胞凋亡率也逐渐上升;马钱子碱400μg/ml作用72小时后的K562细胞出现细胞凋亡的典型梯形条带。结论 :马钱子碱能有效抑制K562细胞生长,诱导其凋亡,并且在50-400μg/ml范围内呈剂量效应关系。
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| 关 键 词： | 马钱子碱 白血病 K562细胞 细胞凋亡 |
Apoptosis-inducing effects of brucine on human chronic myeloid leukemia cell line K562 |
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| Wang HL,We W,Ji AF,Shen XL,Zhang GX,Zhang MX,Zhai CY.Apoptosis-inducing effects of brucine on human chronic myeloid leukemia cell line K562[J].Journal of Experimental Hematology,2011,19(3):630-633. |
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| Authors: | Wang Hai-Li We Wu Ji Ai-Fan Shen Xu-Liang Zhang Guo-Xiang Zhang Mei-Xiang Zhai Chun-Yan |
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| Institution: | WANG Hai-Li,WE Wu1,JI Ai-Fan1,SHEN Xu-Liang1,ZHANG Guo-Xiang1,ZHANG Mei-Xiang1,ZHAI Chun-Yan1 First Clinical Department,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China,1Peace Hospital Affilated to Changzhi Medical College,Changzhi 046000 |
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| Abstract: | To investigate the apoptosis-induction effect of brucine on human chronic myeloid leukemia cell line K562 cells,K562 cells were exposed to various dosages of brucine.MTT method was used to assayed the growth inhibition effect of brucine on K562 cells.The apoptosis of K562 cells was detected by acridine orange/ethidium bromide(AO/EB)double staining,Annexin-V/PI double labeling method and DNA agarose gel elelectrophoresis.The results showed that brucine could remarkably inhibit the K562 cell growth in a conce... |
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| Keywords: | brucine leukemia K562 cell apoptosis |
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