| 不同浓度硼替佐米对柔红霉素诱导的K562耐药细胞株ERK、JNK及P38表达的影响 |
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| 作者姓名： | Fu BB Fan Y Hao LC Liao AJ Liu ZG |
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| 作者单位： | 1. 中国医科大学盛京医院血液病治疗中心,辽宁沈阳,110022
2. 中国医科大学盛京医院第一微创外科,辽宁沈阳,110004 |
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| 摘 要： | 本研究旨在观察蛋白酶体抑制剂硼替佐米(bortezomib,PS-341)对柔红霉素(daunorubicin,DNR)诱导的K562白血病耐药细胞株ERK、JNK及P38表达的影响,探讨硼替佐米逆转耐药的分子机制。用四甲基偶氮唑盐微量酶反应比色法(MTT法)进行耐药细胞株和硼替佐米细胞毒性的判定。以100nmol/L DNR单用或联合应用1及10nmol/L PS-341作用于K562耐药细胞株36小时,检测各组ERK、JNK及P38的表达情况,检测各组细胞凋亡率。结果表明:与DNR组相比,PS-341联合DNR可抑制ERK和P38的表达,增加JNK的表达(p<0.05)。结论 :PS-341可显著抑制K562耐药细胞株ERK和P38的表达,增加JNK的表达;PS-341通过MAPK途径逆转细胞耐药,促进细胞凋亡。
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| 关 键 词： | 硼替佐米 柔红霉素 K562细胞 ERK JNK P38 |
Effect of different concentrations of bortezomib on the expression of ERK, JNK and P38 in daunorubicin-resistant K562 cells |
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| Fu BB,Fan Y,Hao LC,Liao AJ,Liu ZG.Effect of different concentrations of bortezomib on the expression of ERK, JNK and P38 in daunorubicin-resistant K562 cells[J].Journal of Experimental Hematology,2011,19(3):671-675. |
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| Authors: | Fu Bei-Bei Fan Ying Hao Liang-Chun Liao Ai-Jun Liu Zhuo-Gang |
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| Institution: | FU Bei-Bei,FAN Ying1,HAO Liang-Chun,LIAO Ai-Jun,LIU Zhuo-Gang Department of Hematology,China Medical University Shengjing Hospital,Shenyang 110022,Liaoning Province,China,1Department of First Minimal Invasive Surgery,Shenyang 110004 |
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| Abstract: | The aim of this study was to investigate the effect of proteasome inhibitor bortezomib on the expression of ERK,JNK,and P38 in daunorubicin(DNR)-resistant K562 cells and its mechanism.MTT method was used to determine the durg-resistant K562 cells and the cellular toxicity of bortezomib;Western blot was used to detect the expression of protein ERK,JNK and P38 in K562 cells after treatment with 100 nmol/L DNR alone or combined with 1 nmol/L and 10 nmol/L bortezomib for 36 hours.Flow cytometry assay was used t... |
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| Keywords: | bortezomib daunorubicin K562 cell ERK JNK P38 |
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