富氧水的制备、稳定性及其对小鼠血液毒性的影响

目的研究自制富氧水的稳定性及其对小鼠血液的毒性影响。方法监测水的不同体积、是否提前通气、是否加压等条件下利用简易富氧水发生装置制得的富氧水浓度及其变化;以4 mg/kg和2.4 mg/kg的剂量通过灌胃给予小鼠富氧水30 d后,检测电解质水平和相关血液指标。结果研究表明,水的体积越小、提前通入氧气以及外加氧压等方式可以得到初始氧含量较高的富氧水(P<0.05);纯水体积的大小既可影响所得富氧水初始氧含量,也可影响富氧水中氧含量的下降速度;与开放存放相比,密闭存放可减缓氧含量的下降速度。小鼠血液毒性研究表明,以4 mg/kg剂量给予富氧水的小鼠红细胞数量、血红蛋白、红细胞压积等指标都显著降低(P<0.05),而平均红细胞血红蛋白含量等指标显著高于对照组(P<0.05)。结论富氧水的氧含量稳定性随着制备条件和贮存方法的不同而异;高剂量富氧水可引起红细胞携氧功能的降低。

Preparation of Oxygenized Water and Its Stability and Toxicity on Mice Blood

Objective To study the stability of oxygenized water and its toxicological effect on mice blood.Methods Monitoring the contents and changes of oxygen in oxygenized water prepared by simple devices on different conditions,such as different volume of water used,whether the water was ventilated ahead and whether the oxygen pressure was put on the water during the process of oxygenation;after administering oxygenized water at doses of 4 mg/kg and 2.4 mg/kg for 30 days,the mice were sacrificed to detect the related blood indexes.Results Research showed that the content of oxygen in oxygenized water was much higher if it was prepared with small quantity of ventilated water which was put on with foreign oxygen pressure during the process of preparation,in addition,the volume of water used to get oxygenized water could not only affect the initial oxygen content,but also affect the decline rate of oxygen content in oxygenized water.we also found that the oxygen content of oxygenized water would remain at high level for several days if it be preserved under sealed condition.Mice blood toxicity research showed that the values of WBC and HB reduced significantly besides other indexes(P<0.05),and the values of some indexes,such as MCH and MCHC,increase significantly when compared with the counterparts of the control group(P<0.05).Conclusion The content of oxygen in oxygenized water can vary on different conditions of preparation and preservation;and high dose oxygenized water has adverse effects on blood system,which will reduce the oxygen absorption in mice.