| 不同化学染色法对日本血吸虫虫卵染色的效果分析 |
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| 引用本文： | 丘继哲,邹艳,曾庆仁,蔡力汀,黄旭,徐妮为.不同化学染色法对日本血吸虫虫卵染色的效果分析[J].中国病原生物学杂志,2010(4). |
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| 作者姓名： | 丘继哲 邹艳 曾庆仁 蔡力汀 黄旭 徐妮为 |
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| 作者单位： | 湖南中医药高等专科学校基础医学部;中南大学湘雅医学院寄生虫学系; |
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| 基金项目： | 湖南省卫生厅资助项目(No.B2008-068) |
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| 摘 要： | 目的探索不同化学染色法对日本血吸虫虫卵染色的效果,以期获得在镜检下可分辨虫卵和粪渣的染色方法,为粪检血吸虫虫卵的计算机自动化识别奠定基础。方法用AO-EB、罗丹明、中性红、酸性品红、甲苯胺兰、台盼蓝、考马斯亮蓝和孔雀绿等化学染料,选择3个不同浓度和2个不同温度条件,分别对含日本血吸虫虫卵粪液进行染色、涂片镜检,用虫卵和粪渣反差程度作为主要评价指标,比较各种方法的染色效果。结果在8种化学染色方法中,以AO-EB染色和中性红染色的虫卵与粪渣其反差最大,镜下粪涂片中虫卵易于辨认,AO-EB法和中性红法的检出率分别为67.5%和65.0%,明显高于改良加藤法的检出率(40.0%)。结论AO-EB法和中性红染色法有助于提高粪检血吸虫虫卵检出率。
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| 关 键 词： | 血吸虫 日本 虫卵 化学染色 效果 |
Analysis of the effectiveness of different chemical dyes at staining Schistosoma japonicum eggs |
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| QIU Ji-zhe,ZOU Yan,ZENG Qing-ren,CAI Li-ting,HUANG Xu,XU Ni-wei.Analysis of the effectiveness of different chemical dyes at staining Schistosoma japonicum eggs[J].Journal of Pathogen Biology,2010(4). |
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| Authors: | QIU Ji-zhe ZOU Yan ZENG Qing-ren CAI Li-ting HUANG Xu XU Ni-wei |
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| Institution: | QIU Ji-zhe1,ZOU Yan1,ZENG Qing-ren2,CAI Li-ting2,HUANG Xu1,XU Ni-wei1(1.Hunan College of Traditional Chinese Medicine,Zhuzhou 412012,Hunan,China,2.Experimental Center for Cellular & Molecular Biology,Xiangya School of Medicine,Central South University,Changsha 410013,China) |
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| Abstract: | Objective To examine the effectiveness of different chemical dyes at staining Schistosoma japonicum eggs in order to provide a method of staining that can easily distinguish eggs and fecal residue under a microscope.This may have considerable significance for the automated identification of schistosome eggs.Methods At different concentrations and temperatures,S.japonicum eggs in liquid feces were stained with the following chemical dyes:AO-EB,rhodamine,neutral red,acid fuchsin,toluidine blue,trypan blue,Coo... |
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| Keywords: | Schistosoma japonicum eggs chemical staining stool examination |
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